Additional file 3: of Species-level bacterial community profiling of the healthy sinonasal microbiome using Pacific Biosciences sequencing of full-length 16S rRNA genes

Figure S1. Effect of primary filters on the number of reads. Figure S2. Insert size distribution. Figure S3. Effects of Host DNA on bacterial 16S yield. Figure S4. Total CCS yield vs. DNA yield vs. PCR yields. Figure S5. CCS reads mapping to the human genome from the sinonasal samples. Figure S6. Primer matching filters. Figure S7. Primer matching truncation and nucleotide variability against positive control E.coli forward and reverse primer matches. Figure S8. Primer matching truncation and nucleotide variability against positive control A.tumefaciens forward and reverse primer matches. Figure S9. Histogram of the number of species per DB cluster in NCBI. Figure S10. The effect of PCR cycle number and polymerase choice on OTU abundances in the BEI mock community. Figure S11. The effect of PCR cycles and polymerase on abundances of chimeric molecules in the BEI mock community. Figure S12. Substitution errors in BEI mock community. Figure S13. E.coli MG1655 16S copies and MED analysis. Figure S14. Phylogenetic trees of well---resolved multi---species genera Clostridium and Desulfovibrio. Figure S15. Phylogenetic trees of poorly---resolved multi---species genera Azotobacter and Nonlabens. Figure S16. Phylogenetic trees of multi---species genera with improved species resolution using FL16S for Algoriphagus and Salegentibacter. Figure S17. Phylogeny of AnaerococcusMED nodes from the sinonasal communities plus NCBI database entries. Figure S18. Relationship between species---level confidence in centroid assignments and the number of species in the matching dbOTU. Figure S19. Effective Number of Species as a function of sample read depth. (PDF 3167 kb)