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Lipid analysis by ion mobility spectrometry combined with mass spectrometry: A brief update with a perspective on applications in the clinical laboratory
- Source :
- Journal of Mass Spectrometry and Advances in the Clinical Lab, Journal of Mass Spectrometry and Advances in the Clinical Lab, Vol 23, Iss, Pp 7-13 (2022)
- Publication Year :
- 2021
- Publisher :
- Elsevier, 2021.
-
Abstract
- Ion mobility spectrometry (IMS) is an analytical technique where ions are separated in the gas phase based on their mobility through a buffer gas in the presence of an electric field. An ion passing through an IMS device has a characteristic collisional cross section (CCS) value that depends on the buffer gas used. IMS can be coupled with mass spectrometry (MS), which characterizes an ion based on a mass-to-charge ratio (m/z), to increase analytical specificity and provide further physicochemical information. In particular, IMS-MS is of ever-increasing interest for the analysis of lipids, which can be problematic to accurately identify and quantify in bodily fluids by liquid chromatography (LC) with MS alone due to the presence of isomers, isobars, and structurally similar analogs. IMS provides an additional layer of separation when combined with front-end LC approaches, thereby, enhancing peak capacity and analytical specificity. CCS (and also ion mobility drift time) can be plotted against m/z ion intensity and/or LC retention time in order to generate in-depth molecular profiles of a sample. Utilization of IMS-MS for routine clinical laboratory testing remains relatively unexplored, but areas do exist for potential implementation. A brief update is provided here on lipid analysis using IMS-MS with a perspective on some applications in the clinical laboratory.
- Subjects :
- PC, glycerophosphocholine
TIMS, trapped ion mobility spectrometry
MALDI, matrix-assisted laser desorption/ionization
Clinical Biochemistry
FIA, flow injection analysis
TWIMS, traveling wave ion mobility spectrometry
CVD, cardiovascular disease
Clinical analysis
PE, phosphatidylethanolamine
PG, phosphatidylglycerol
TOF, time-of-flight
Microbiology
Special issue on Lipidomics
NBS, newborn screening
DMS, differential mobility spectrometry
SLIM, structures for loss less ion manipulations
HRMS, high-resolution mass spectrometry
Medical technology
DG, diacylglycerol
FTICR, fourier-transform ion cyclotron resonance
SV, separation voltage
R855-855.5
CV, compensation voltage
RF, radio frequency
Spectroscopy
DTIMS, drift tube ion mobility spectrometry
LPC, lysophosphatidylcholine
SM, sphingomyelin
TG, triglyceride
Mass spectrometry
LC, liquid chromatography
IMS, ion mobility spectrometry
VLDL, very-low-density lipoprotein
CCS, collisional cross section
FAIMS, field asymmetric waveform ion mobility spectrometry
m/z, mass-to-charge ratio
Ion mobility spectrometry
HDL, high-density-lipoprotein
LDL, low-density-lipoprotein
Lipids
EV, elution voltage
Medical Laboratory Technology
MS, mass spectrometry
IMS-MS, ion mobility spectrometry-mass spectrometry
Subjects
Details
- Language :
- English
- ISSN :
- 2667145X and 26671468
- Volume :
- 23
- Database :
- OpenAIRE
- Journal :
- Journal of Mass Spectrometry and Advances in the Clinical Lab
- Accession number :
- edsair.doi.dedup.....2ae14ffaafac42c15bc615e2dc76a45d