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Simultaneous measurement of RBC velocity, flux, hematocrit and shear rate in vascular networks
- Source :
- Nature methods
- Publication Year :
- 2010
- Publisher :
- Springer Science and Business Media LLC, 2010.
-
Abstract
- Not all tumor vessels are equal. Tumor-associated vasculature includes immature vessels, regressing vessels, transport vessels undergoing arteriogenesis and peritumor vessels influenced by tumor growth factors. Current techniques for analyzing tumor blood flow do not discriminate between vessel subtypes and only measure average changes from a population of dissimilar vessels. We developed methodologies for simultaneously quantifying blood flow (velocity, flux, hematocrit and shear rate) in extended networks at single-capillary resolution in vivo. Our approach relies on deconvolution of signals produced by labeled red blood cells as they move relative to the scanning laser of a confocal or multiphoton microscope and provides fully resolved three-dimensional flow profiles within vessel networks. Using this methodology, we show that blood velocity profiles are asymmetric near intussusceptive tissue structures in tumors in mice. Furthermore, we show that subpopulations of vessels, classified by functional parameters, exist in and around a tumor and in normal brain tissue.
- Subjects :
- Erythrocytes
Confocal
Population
Hematocrit
Biochemistry
Article
Microcirculation
Mice
03 medical and health sciences
0302 clinical medicine
Neoplasms
medicine
Animals
education
Molecular Biology
030304 developmental biology
0303 health sciences
education.field_of_study
medicine.diagnostic_test
Chemistry
Cell Biology
Blood flow
Anatomy
Shear rate
Hemorheology
cardiovascular system
Arteriogenesis
Blood Flow Velocity
030217 neurology & neurosurgery
Biotechnology
Biomedical engineering
Subjects
Details
- ISSN :
- 15487105 and 15487091
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Nature Methods
- Accession number :
- edsair.doi.dedup.....2afd2396ac6ecb03bd41fa1555af64f5