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The importance of oxygen free radicals in the etiopathogenesis of diversion colitis in rats
- Source :
- Acta Cirúrgica Brasileira v.25 n.5 2010, Acta Cirúrgica Brasileira, Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC), instacron:SBDPC, Acta Cirurgica Brasileira, Volume: 25, Issue: 5, Pages: 387-395, Published: OCT 2010
- Publication Year :
- 2010
-
Abstract
- PURPOSE: Quantify the levels of oxidative DNA damage of epithelial colon cells comparing segments with and without fecal stream. METHODS: Sixty Wistar rats were subjected to deviation of fecal stream by proximal colostomy and a distal mucosal fistula. Animals were divided into three experimental groups that were sacrificed 6, 12 and 24 weeks after surgery. In each experimental group, five animals underwent laparotomy without intestinal deviation (sham subgroup). The diagnosis of colitis was made by histopathological analysis and the inflammatory activity index by graduated scale. The neutrophil infiltration was determined by myeloperoxidase tissue levels and the intensity of oxidative DNA damage by comet assay. The Mann-Withney and Student t test were used to compare the results among experimental subgroups and the Kruskal-Wallis test for variance analysis, adopting a significance level of 5% (p
- Subjects :
- Male
medicine.medical_specialty
Free Radicals
DNA damage
medicine.medical_treatment
medicine.disease_cause
Gastroenterology
Statistics, Nonparametric
Feces
Random Allocation
Dano ao DNA
Internal medicine
Colostomy
medicine
Animals
Ensaio em Cometa
Colitis
Intestinal Mucosa
Rats, Wistar
Gastrointestinal Transit
Diversion colitis
Ratos
Peroxidase
biology
business.industry
Estresse Oxidativo
Colostomia
Ácidos Graxos Voláteis
Fatty Acids, Volatile
medicine.disease
Rats
Comet assay
Disease Models, Animal
Oxidative Stress
Myeloperoxidase
biology.protein
Colite
Surgery
Comet Assay
Analysis of variance
business
Oxidative stress
DNA Damage
Subjects
Details
- ISSN :
- 16782674
- Volume :
- 25
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Acta cirurgica brasileira
- Accession number :
- edsair.doi.dedup.....2b134dfaf5f434b23a0866757665685e