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A simple and sensitive method for determining plasma cell isotype and monoclonality in bone marrow using flowcytometry
- Source :
- British journal of haematology. 91(1)
- Publication Year :
- 1995
-
Abstract
- In this paper we describe a new, rapid and sensitive method to determine plasma cell isotype and clonality in bone marrow using flowcytometry. With the use of a new fixation and permeabilization reagent (Permeafix), which preserves cell structure and morphology, and a monoclonal antibody (Mab) specific for plasma cells (B-B4), it has become possible to specifically select plasma cells and to determine the cytoplasmatic immunoglobulins by flowcytometry. Thirty successive bone marrow aspirates from multiple myeloma patients and patients with MGUS were studied as well as 10 bone marrow samples from patients with reactive plasmacytosis. Each sample was analysed both by immunofluorescence on cytospin smears and FACS analysis. There were no discrepancies between plasma cell isotype as determined by FACS and cytospin. Moreover, FACS analysis was shown to allow detection of very low numbers of plasma cells and to determine whether these plasma cells are mono- or polyclonal. Possible applications are discussed.
- Subjects :
- Pathology
medicine.medical_specialty
Cytoplasm
medicine.drug_class
Plasma Cells
Cell Count
Plasma cell
Monoclonal antibody
Immunofluorescence
Antibodies
Immunophenotyping
Fixatives
Bone Marrow
medicine
Humans
Multiple myeloma
biology
medicine.diagnostic_test
Plasmacytosis
Hematology
medicine.disease
Flow Cytometry
Molecular biology
Isotype
Clone Cells
Immunoglobulin Isotypes
medicine.anatomical_structure
biology.protein
Bone marrow
Antibody
Multiple Myeloma
Subjects
Details
- ISSN :
- 00071048
- Volume :
- 91
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- British journal of haematology
- Accession number :
- edsair.doi.dedup.....2b1dac9383d73f972796c04f165641b7