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Structure, Dynamics, Evolution, and Function of a Major Scaffold Component in the Nuclear Pore Complex
- Source :
- Structure (London, England : 1993), vol 21, iss 4
- Publisher :
- Elsevier Ltd.
-
Abstract
- Summary The nuclear pore complex, composed of proteins termed nucleoporins (Nups), is responsible for nucleocytoplasmic transport in eukaryotes. Nuclear pore complexes (NPCs) form an annular structure composed of the nuclear ring, cytoplasmic ring, a membrane ring, and two inner rings. Nup192 is a major component of the NPC's inner ring. We report the crystal structure of Saccharomyces cerevisiae Nup192 residues 2–960 [ScNup192(2–960)], which adopts an α-helical fold with three domains (i.e., D1, D2, and D3). Small angle X-ray scattering and electron microscopy (EM) studies reveal that ScNup192(2–960) could undergo long-range transition between "open" and "closed" conformations. We obtained a structural model of full-length ScNup192 based on EM, the structure of ScNup192(2–960), and homology modeling. Evolutionary analyses using the ScNup192(2–960) structure suggest that NPCs and vesicle-coating complexes are descended from a common membrane-coating ancestral complex. We show that suppression of Nup192 expression leads to compromised nuclear transport and hypothesize a role for Nup192 in modulating the permeability of the NPC central channel.
- Subjects :
- Small Angle
Models, Molecular
Saccharomyces cerevisiae Proteins
Evolution
Protein Conformation
1.1 Normal biological development and functioning
Biophysics
Active Transport, Cell Nucleus
Saccharomyces cerevisiae
Biology
Electron
Article
Scattering
Evolution, Molecular
03 medical and health sciences
0302 clinical medicine
Protein structure
Models
Underpinning research
Structural Biology
Information and Computing Sciences
Scattering, Small Angle
Homology modeling
Nuclear pore
Molecular Biology
030304 developmental biology
Cell Nucleus
Microscopy
0303 health sciences
Molecular
Biological Sciences
Active Transport
Transport protein
Nuclear Pore Complex Proteins
Microscopy, Electron
Crystallography
Cytoplasm
Nucleocytoplasmic Transport
Chemical Sciences
Nuclear Pore
Generic health relevance
Nucleoporin
Nuclear transport
Crystallization
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 09692126
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Structure
- Accession number :
- edsair.doi.dedup.....2b2a977b54861cb00459fa05bf833d5f
- Full Text :
- https://doi.org/10.1016/j.str.2013.02.005