Additional file 1 of Comparisons within the Rice GA 2-Oxidase Gene Family Revealed Three Dominant Paralogs and a Functional Attenuated Gene that Led to the Identification of Four Amino Acid Variants Associated with GA Deactivation Capability

Additional file 1 : Figure S1. Phylogenetic tree based on comparisons of OsGA2ox amino acid sequences. Figure S2. Effects of exogenous GA3 treatment on seedling growth. Figure S3. Relative positions of amino acids Q220 and Y274 in OsGA2ox3 according to the resolved 3-D protein structure. Figure S4. Phenotypic comparison of WT-OX, 3E-OX, 3F-OX and 3EF-OX transgenic rice plants and their RNA expression analysis. Figure S5. Phenotypic comparison of WT-OX, 3R-OX, 3P-OX and 3RP-OX transgenic rice plants and their RNA expression analysis. Figure S6. Locations of and evolutionary relationships among the OsGA2ox genes on rice chromosomes. Figure S7. Expression analysis of OsGA2ox genes in various tissues according to data collected from different rice expression databases. Figure S8. Phenotypic comparisons of CRISPR/Cas9 knockout OsGA2ox1, OsGA2ox3, and OsGA2ox6 mutants. Table S1A. The locations and aligned lengths of the two conserved regions in the first intron of OsGA2ox1 and its orthologs in Bd and Sb. Table S1B. The locations and aligned lengths of the conserved regions in the 5′-regulatory region of OsGA2ox3 and its orthologs in Bd and Sb. Table S1C. The locations and aligned lengths of the conserved region in the first intron of OsGA2ox6 and its orthologs in Bd and Sb. Table S2A. DNA-binding motifs in the first intron region of OsGA2ox1. Table S2B. DNA-binding motifs in promoter regions of OsGA2ox3. Table S2C. DNA-binding motifs in the first intron region of OsGA2ox6. Table S3. List of primers and their sequences used in this study.