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CYCLING DOF FACTOR 1 represses transcription through the TOPLESS co-repressor to control photoperiodic flowering in Arabidopsis
- Source :
- The Plant Journal. 92:244-262
- Publication Year :
- 2017
- Publisher :
- Wiley, 2017.
-
Abstract
- Summary CYCLING DOF FACTOR 1 (CDF1) and its homologs play an important role in the floral transition by repressing the expression of floral activator genes such as CONSTANS (CO) and FLOWERING LOCUS T (FT) in Arabidopsis. The day-length-specific removal of CDF1-dependent repression is a critical mechanism in photoperiodic flowering. However, the mechanism by which CDF1 represses CO and FT transcription remained elusive. Here we demonstrate that Arabidopsis CDF proteins contain non-EAR motif-like conserved domains required for interaction with the TOPLESS (TPL) co-repressor protein. This TPL interaction confers a repressive function on CDF1, as mutations of the N-terminal TPL binding domain largely impair the ability of CDF1 protein to repress its targets. TPL proteins are present on specific regions of the CO and FT promoters where CDF1 binds during the morning. In addition, TPL binding increases when CDF1 expression is elevated, suggesting that TPL is recruited to these promoters in a time-dependent fashion by CDFs. Moreover, reduction of TPL activity induced by expressing a dominant negative version of TPL (tpl-1) in phloem companion cells results in early flowering and a decreased sensitivity to photoperiod in a manner similar to a cdf loss-of-function mutant. Our results indicate that the mechanism of CDF1 repression is through the formation of a CDF–TPL transcriptional complex, which reduces the expression levels of CO and FT during the morning for seasonal flowering.
- Subjects :
- 0301 basic medicine
Photoperiod
Mutant
Arabidopsis
Flowers
Plant Science
Article
03 medical and health sciences
Gene Expression Regulation, Plant
Transcription (biology)
Botany
Genetics
Gene
Psychological repression
biology
Arabidopsis Proteins
Activator (genetics)
food and beverages
Promoter
Cell Biology
biology.organism_classification
Cell biology
Repressor Proteins
030104 developmental biology
Co-Repressor Proteins
Binding domain
Subjects
Details
- ISSN :
- 09607412
- Volume :
- 92
- Database :
- OpenAIRE
- Journal :
- The Plant Journal
- Accession number :
- edsair.doi.dedup.....2b5b8aa9724cefd24fb71e791dfdd848