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Alkaline serine proteinase: a major allergen of Aspergillus oryzae and its cross-reactivity with Penicillium citrinum
Alkaline serine proteinase: a major allergen of Aspergillus oryzae and its cross-reactivity with Penicillium citrinum
- Source :
- International archives of allergy and immunology. 116(1)
- Publication Year :
- 1998
-
Abstract
- Background:Aspergillus species are common indoor airborne fungi and have been considered as causative agents of human allergic disorders. However, allergens of different Aspergillus species have not been effectively characterized. The object of this study was to identify and characterize IgE-binding components of Aspergillus oryzae. Methods. Allergens of A. oryzae were identified by immunoblot analysis using sera from asthmatic patients. The N-terminal amino acid sequences of allergens thus identified were determined by Edman degradation. The antigenic and the allergenic cross-reactivities between allergens of different fungi were analyzed by immunoblotting and immunoblot inhibition analysis, respectively, using a monoclonal antibody (MoAb) 55A against the 33-kD major allergen of Penicillium citrinum and a mixture of IgE-containing asthmatic serum samples. Results: Thirteen components of A. oryzae ranging in apparent molecular weight from 16 to 42 kD react with IgE antibodies. A 34-kD component that showed intense IgE-binding reactivity and was detectable in the highest frequency in our asthmatic serum samples tested was considered a major allergen of A. oryzae. The 34-kD component also reacted with MoAb 55A. Results from immunoblot inhibition studies also demonstrated the IgE cross-reactivity between the 34-kD major allergens of A. oryzae and P. citrinum. In addition, the sequence of the N-terminal 18 amino acid residues of the 34-kD major allergen of A. oryzae was found to be identical to that of the alkaline serine proteinase from the same Aspergillus species. Conclusion: The 34-kD major allergen of A. oryzae is an alkaline serine proteinase. There is IgE cross-reactivity between the major serine proteinase allergens of A. oryzae and P. citrinum.
- Subjects :
- Allergy
Antigens, Fungal
Aspergillus oryzae
Immunology
Immunoblotting
Molecular Sequence Data
Cross Reactions
medicine.disease_cause
Cross-reactivity
Binding, Competitive
Microbiology
Serine
Fungal Proteins
chemistry.chemical_compound
Allergen
medicine
Immunology and Allergy
Humans
Electrophoresis, Gel, Two-Dimensional
Penicillium citrinum
Amino Acid Sequence
Binding Sites
biology
Sequence Homology, Amino Acid
Serine Endopeptidases
Penicillium
Aeroallergen
General Medicine
Fungi imperfecti
Allergens
Immunoglobulin E
biology.organism_classification
medicine.disease
Asthma
Antibodies, Anti-Idiotypic
chemistry
Biochemistry
Sequence Analysis
Protein Binding
Subjects
Details
- ISSN :
- 10182438
- Volume :
- 116
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- International archives of allergy and immunology
- Accession number :
- edsair.doi.dedup.....2b683be0473e8d030dbe5612c08886df