Back to Search
Start Over
Evaluating Antibody Mediated Protection against Alpha, Beta, and Delta SARS-CoV-2 Variants of Concern in K18-hACE2 Transgenic Mice
Evaluating Antibody Mediated Protection against Alpha, Beta, and Delta SARS-CoV-2 Variants of Concern in K18-hACE2 Transgenic Mice
- Source :
- Journal of Virology. 96
- Publication Year :
- 2022
- Publisher :
- American Society for Microbiology, 2022.
-
Abstract
- SARS-CoV-2 variants of concern (VoC) are impacting responses to the COVID-19 pandemic. Here, we utilized passive immunization using human convalescent plasma (HCP) obtained from a critically ill COVID-19 patient in the early pandemic to study the efficacy of polyclonal antibodies generated to ancestral SARS-CoV-2 against the Alpha, Beta, and Delta VoC in the K18 human angiotensin converting enzyme 2 (hACE2) transgenic mouse model. HCP protected mice from challenge with the original WA-1 SARS-CoV-2 strain; however, only partially protected mice challenged with the Alpha VoC (60% survival) and failed to save Beta challenged mice from succumbing to disease. HCP treatment groups had elevated receptor binding domain (RBD) and nucleocapsid IgG titers in the serum; however, Beta VoC viral RNA burden in the lung and brain was not decreased due to HCP treatment. While mice could be protected from WA-1 or Alpha challenge with a single dose of HCP, six doses of HCP could not decrease mortality of Delta challenged mice. Overall, these data demonstrate that VoC have enhanced immune evasion and this work underscores the need for
- Subjects :
- SARS-CoV-2
Immunology
Immunization, Passive
COVID-19
Mice, Transgenic
biochemical phenomena, metabolism, and nutrition
Antibodies, Neutralizing
Microbiology
Disease Models, Animal
Mice
Virology
Insect Science
Animals
Humans
Angiotensin-Converting Enzyme 2
gamma-Globulins
Melphalan
COVID-19 Serotherapy
Subjects
Details
- ISSN :
- 10985514 and 0022538X
- Volume :
- 96
- Database :
- OpenAIRE
- Journal :
- Journal of Virology
- Accession number :
- edsair.doi.dedup.....2b79849e969f4db9ec0604d47bf75cdd
- Full Text :
- https://doi.org/10.1128/jvi.02184-21