Back to Search Start Over

Longer Duration of Active Oil Biosynthesis during Seed Development Is Crucial for High Oil Yield—Lessons from Genome-Wide In Silico Mining and RNA-Seq Validation in Sesame

Authors :
Bhagwat Nawade
Ajay Kumar
Rasna Maurya
Rajkumar Subramani
Rashmi Yadav
Kuldeep Singh
Parimalan Rangan
Source :
Plants; Volume 11; Issue 21; Pages: 2980
Publication Year :
2022
Publisher :
MDPI AG, 2022.

Abstract

Sesame, one of the ancient oil crops, is an important oilseed due to its nutritionally rich seeds with high protein content. Genomic scale information for sesame has become available in the public databases in recent years. The genes and their families involved in oil biosynthesis in sesame are less studied than in other oilseed crops. Therefore, we retrieved a total of 69 genes and their translated amino acid sequences, associated with gene families linked to the oil biosynthetic pathway. Genome-wide in silico mining helped identify key regulatory genes for oil biosynthesis, though the findings require functional validation. Comparing sequences of the SiSAD (stearoyl-acyl carrier protein (ACP)-desaturase) coding genes with known SADs helped identify two SiSAD family members that may be palmitoyl-ACP-specific. Based on homology with lysophosphatidic acid acyltransferase (LPAAT) sequences, an uncharacterized gene has been identified as SiLPAAT1. Identified key regulatory genes associated with high oil content were also validated using publicly available transcriptome datasets of genotypes contrasting for oil content at different developmental stages. Our study provides evidence that a longer duration of active oil biosynthesis is crucial for high oil accumulation during seed development. This underscores the importance of early onset of oil biosynthesis in developing seeds. Up-regulating, identified key regulatory genes of oil biosynthesis during early onset of seed development, should help increase oil yields.

Details

ISSN :
22237747
Volume :
11
Database :
OpenAIRE
Journal :
Plants
Accession number :
edsair.doi.dedup.....2b8800fb218f5ab9d0eceacacbaaeb59
Full Text :
https://doi.org/10.3390/plants11212980