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Novel electrospun poly-hydroxybutyrate scaffolds as carriers for the wound healing agents alkannins and shikonins
- Source :
- Regenerative Biomaterials, REGENERATIVE BIOMATERIALS
- Publication Year :
- 2021
- Publisher :
- Oxford University Press, 2021.
-
Abstract
- The aim of this study was to investigate the potential of novel electrospun fiber mats loaded with alkannin and shikonin (A/S) derivatives, using as carrier a highly biocompatible, bio-derived, eco-friendly polymer such as poly[(R)-3-hydroxybutyric acid] (PHB). PHB fibers containing a mixture of A/S derivatives at different ratios were successfully fabricated via electrospinning. Αs evidenced by scanning electron microscopy, the fibers formed a bead-free mesh with average diameters from 1.25 to 1.47 μm. Spectroscopic measurements suggest that electrospinning marginally increases the amorphous content of the predominantly crystalline PHB in the fibers, while a significant drug amount lies near the fiber surface for samples of high total A/S content. All scaffolds displayed satisfactory characteristics, with the lower concentrations of A/S mixture-loaded PHB fiber mats achieving higher porosity, water uptake ratios, and entrapment efficiencies. The in vitro dissolution studies revealed that all samples released more than 70% of the encapsulated drug after 72 h. All PHB scaffolds tested by cell viability assay were proven non-toxic for Hs27 fibroblasts, with the 0.15 wt.% sample favoring cell attachment, spreading onto the scaffold surface, as well as cell proliferation. Finally, the antimicrobial activity of PHB meshes loaded with A/S mixture was documented for Staphylococcus epidermidis and S. aureus.
- Subjects :
- DRUG-RELEASE
Biocompatibility
wound
FABRICATION
BIOLOGY
wound healing
02 engineering and technology
CONTROLLED-RELEASE
010402 general chemistry
01 natural sciences
Polyhydroxyalkanoates
Biomaterials
chemistry.chemical_compound
DELIVERY
Cell-matrix adhesion
CHEMISTRY
Medicine and Health Sciences
Viability assay
Fiber
electrospinning
chemistry.chemical_classification
Alkannin
shikonin
polyhydroxyalkanoates
technology, industry, and agriculture
Biology and Life Sciences
Polymer
NANOFIBROUS MATS
alkannin
021001 nanoscience & nanotechnology
healing
FIBER MEMBRANES
Electrospinning
0104 chemical sciences
chemistry
Chemical engineering
SOLUTE RELEASE
AcademicSubjects/SCI01410
0210 nano-technology
AcademicSubjects/MED00010
BEHAVIOR
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 20563426 and 20563418
- Volume :
- 8
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Regenerative Biomaterials
- Accession number :
- edsair.doi.dedup.....2b88cb545991c14e269bc2a8222e888d