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Bone Marrow Stem Cells and Ear Framework Reconstruction

Authors :
Mohammad-Kazem Olad-Gubad
Hamid Karimi
Seyed-Abolhassan Emami
Source :
Journal of Craniofacial Surgery. 27:2192-2196
Publication Year :
2016
Publisher :
Ovid Technologies (Wolters Kluwer Health), 2016.

Abstract

Repair of total human ear loss or congenital lack of ears is one of the challenging issues in plastic and reconstructive surgery.The aim of the present study was 3D reconstruction of the human ear with cadaveric ear cartilages seeded with human mesenchymal stem cells.We used cadaveric ear cartilages with preserved perichondrium. The samples were divided into 2 groups: group A (cartilage alone) and group B (cartilage seeded with a mixture of fibrin powder and mesenchymal stem cell [1,000,000 cells/cm] used and implanted in back of 10 athymic rats). After 12 weeks, the cartilages were removed and shape, size, weight, flexibility, and chondrocyte viability were evaluated. P value0.05 was considered significant.In group A, size and weight of cartilages clearly reduced (P 0.05) and then shape and flexibility (torsion of cartilages in clockwise and counterclockwise directions) were evaluated, which were found to be significantly reduced (P 0.05). After staining with hematoxylin and eosin and performing microscopic examination, very few live chondrocytes were found in group A. In group B, size and weight of samples were not changed (P 0.05); the shape and flexibility of samples were well maintained (P 0.05) and on performing microscopic examination of cartilage samples, many live chondrocytes were found in cartilage (15-20 chondrocytes in each microscopic field).In samples with human stem cell, all variables (size, shape, weight, and flexibility) were significantly maintained and abundant live chondrocytes were found on performing microscopic examination. This method may be used for reconstruction of full defect of auricles in humans.

Details

ISSN :
10492275
Volume :
27
Database :
OpenAIRE
Journal :
Journal of Craniofacial Surgery
Accession number :
edsair.doi.dedup.....2b890e9721b1bed67d0695a093e35339
Full Text :
https://doi.org/10.1097/scs.0000000000003146