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Bone Marrow Stem Cells and Ear Framework Reconstruction
- Source :
- Journal of Craniofacial Surgery. 27:2192-2196
- Publication Year :
- 2016
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2016.
-
Abstract
- Repair of total human ear loss or congenital lack of ears is one of the challenging issues in plastic and reconstructive surgery.The aim of the present study was 3D reconstruction of the human ear with cadaveric ear cartilages seeded with human mesenchymal stem cells.We used cadaveric ear cartilages with preserved perichondrium. The samples were divided into 2 groups: group A (cartilage alone) and group B (cartilage seeded with a mixture of fibrin powder and mesenchymal stem cell [1,000,000 cells/cm] used and implanted in back of 10 athymic rats). After 12 weeks, the cartilages were removed and shape, size, weight, flexibility, and chondrocyte viability were evaluated. P value0.05 was considered significant.In group A, size and weight of cartilages clearly reduced (P 0.05) and then shape and flexibility (torsion of cartilages in clockwise and counterclockwise directions) were evaluated, which were found to be significantly reduced (P 0.05). After staining with hematoxylin and eosin and performing microscopic examination, very few live chondrocytes were found in group A. In group B, size and weight of samples were not changed (P 0.05); the shape and flexibility of samples were well maintained (P 0.05) and on performing microscopic examination of cartilage samples, many live chondrocytes were found in cartilage (15-20 chondrocytes in each microscopic field).In samples with human stem cell, all variables (size, shape, weight, and flexibility) were significantly maintained and abundant live chondrocytes were found on performing microscopic examination. This method may be used for reconstruction of full defect of auricles in humans.
- Subjects :
- Adult
Bone Marrow Cells
02 engineering and technology
Chondrocyte
Young Adult
03 medical and health sciences
Chondrocytes
0302 clinical medicine
Tissue engineering
Ear Cartilage
Cadaver
medicine
Animals
Humans
Perichondrium
Ear Auricle
Cells, Cultured
Wound Healing
Tissue Engineering
business.industry
Cartilage
Mesenchymal stem cell
Ear Deformities, Acquired
Bone Marrow Stem Cell
Mesenchymal Stem Cells
General Medicine
Anatomy
Middle Aged
Plastic Surgery Procedures
021001 nanoscience & nanotechnology
Rats
medicine.anatomical_structure
Otorhinolaryngology
030220 oncology & carcinogenesis
Surgery
0210 nano-technology
business
Subjects
Details
- ISSN :
- 10492275
- Volume :
- 27
- Database :
- OpenAIRE
- Journal :
- Journal of Craniofacial Surgery
- Accession number :
- edsair.doi.dedup.....2b890e9721b1bed67d0695a093e35339
- Full Text :
- https://doi.org/10.1097/scs.0000000000003146