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A pH-Triggered, Self-Assembled, and Bioprintable Hybrid Hydrogel Scaffold for Mesenchymal Stem Cell Based Bone Tissue Engineering
- Source :
- ACS Applied Materials & Interfaces
- Publication Year :
- 2019
- Publisher :
- American Chemical Society (ACS), 2019.
-
Abstract
- Effective bone tissue engineering can restore bone and skeletal functions that are impaired by traumas and/or certain medical conditions. Bone is a complex tissue and functions through orchestrated interactions between cells, biomechanical forces, and biofactors. To identify ideal scaffold materials for effective mesenchymal stem cell (MSC)-based bone tissue regeneration, here we develop and characterize a composite nanoparticle hydrogel by combining carboxymethyl chitosan (CMCh) and amorphous calcium phosphate (ACP) (designated as CMCh-ACP hydrogel). We demonstrate that the CMCh-ACP hydrogel is readily prepared by incorporating glucono δ-lactone (GDL) into an aqueous dispersion or rehydrating the acidic freeze-dried nanoparticles in a pH-triggered controlled-assembly fashion. The CMCh-ACP hydrogel exhibits excellent biocompatibility and effectively supports MSC proliferation and cell adhesion. Moreover, while augmenting BMP9-induced osteogenic differentiation, the CMCh-ACP hydrogel itself is osteoinductive and induces the expression of osteoblastic regulators and bone markers in MSCs in vitro. The CMCh-ACP scaffold markedly enhances the efficiency and maturity of BMP9-induced bone formation in vivo, while suppressing bone resorption occurred in long-term ectopic osteogenesis. Thus, these results suggest that the pH-responsive self-assembled CMCh-ACP injectable and bioprintable hydrogel may be further exploited as a novel scaffold for osteoprogenitor-cell-based bone tissue regeneration.
- Subjects :
- Calcium Phosphates
Scaffold
Bone Regeneration
Materials science
Biocompatibility
Biocompatible Materials
02 engineering and technology
BMP9
Bone tissue
Bone and Bones
Bone resorption
Cell Line
03 medical and health sciences
Osteogenesis
Cell Adhesion
medicine
Humans
General Materials Science
bone tissue engineering
Cell adhesion
hydrogels
Cell Proliferation
030304 developmental biology
mesenchymal stem cells
Chitosan
0303 health sciences
Tissue Engineering
Tissue Scaffolds
Regeneration (biology)
Mesenchymal stem cell
amorphous calcium phosphate (ACP)
Bioprinting
Cell Differentiation
Hydrogen-Ion Concentration
021001 nanoscience & nanotechnology
Cell biology
Growth Differentiation Factors
medicine.anatomical_structure
carboxymethyl chitosan (CMCh)
Self-healing hydrogels
Nanoparticles
0210 nano-technology
Research Article
Subjects
Details
- ISSN :
- 19448252 and 19448244
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- ACS Applied Materials & Interfaces
- Accession number :
- edsair.doi.dedup.....2bd82f7f74818a20862c4b9914aa6251