Back to Search
Start Over
High affinity protein surface binding through co-engineering of nanoparticles and proteins
- Source :
- Nanoscale, Nanoscale, vol 14, iss 6, Nanoscale (Online) 14 (2022): 2411–2418. doi:10.1039/d1nr07497k, info:cnr-pdr/source/autori:Ray M.; Brancolini G.; Luther D.C.; Jiang Z.; Cao-Milan R.; Cuadros A.M.; Burden A.; Clark V.; Rana S.; Mout R.; Landis R.F.; Corni S.; Rotello V.M./titolo:High affinity protein surface binding through co-engineering of nanoparticles and proteins/doi:10.1039%2Fd1nr07497k/rivista:Nanoscale (Online)/anno:2022/pagina_da:2411/pagina_a:2418/intervallo_pagine:2411–2418/volume:14
- Publication Year :
- 2022
- Publisher :
- Royal Society of Chemistry (RSC), 2022.
-
Abstract
- Control over supramolecular recognition between proteins and nanoparticles (NPs) is of fundamental importance in therapeutic applications and sensor development. Most NP-protein binding approaches use 'tags' such as biotin or His-tags to provide high affinity; protein surface recognition provides a versatile alternative strategy. Generating high affinity NP-protein interactions is challenging however, due to dielectric screening at physiological ionic strengths. We report here the co-engineering of nanoparticles and protein to provide high affinity binding. In this strategy, 'supercharged' proteins provide enhanced interfacial electrostatic interactions with complementarily charged nanoparticles, generating high affinity complexes. Significantly, the co-engineered protein-nanoparticle assemblies feature high binding affinity even at physiologically relevant ionic strength conditions. Computational studies identify both hydrophobic and electrostatic interactions as drivers for these high affinity NP-protein complexes.
- Subjects :
- Technology
Hydrophobic and Hydrophilic Interactions
Protein Binding
Proteins
Static Electricity
Nanoparticles
Bioengineering
Article
GOLD NANOPARTICLES
ALPHA-CHYMOTRYPSIN
DIFFUSIONAL ASSOCIATION
CYTOSOLIC DELIVERY
SIMULATION
CORONA
RECOGNITION
SIZE
DNA
Physical Sciences
Chemical Sciences
Nanotechnology
General Materials Science
Nanoscience & Nanotechnology
Subjects
Details
- ISSN :
- 20403372 and 20403364
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- Nanoscale
- Accession number :
- edsair.doi.dedup.....2be80cf2b66ffd6f8f5122fbda8bd1df