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Electrochemical assay of the nitrate and nitrite reductase activities of Rhizobium japonicum
- Source :
- Biosensorsbioelectronics. 24(12)
- Publication Year :
- 2009
-
Abstract
- This work describes an electrochemical method for the determination of the nitrate and nitrite reductase activities of Rhizobium japonicum. The advantage of the method lies in the use of whole cells for the analysis and we earlier developed this protocol for the assay of NO. The results obtained are comparable to the spectrophotometric Griess assay. As the method is based on electrochemical reduction, the commonly interfering biological components like ascorbic acid, uric acid, dopamine, etc., will not interfere with the analysis. This method can be extended to the fabrication of biosensors for nitrate and nitrite using the same principle.
- Subjects :
- Nitrite Reductases
Biomedical Engineering
Biophysics
Biosensing Techniques
Nitrate reductase
Nitrate Reductase
Nitric oxide
chemistry.chemical_compound
Nitrate
Electrochemistry
Nitrite
Chromatography
biology
food and beverages
General Medicine
Equipment Design
Ascorbic acid
Nitrite reductase
biology.organism_classification
Amperometry
Enzyme Activation
Equipment Failure Analysis
chemistry
Biochemistry
Biotechnology
Bradyrhizobium japonicum
Rhizobium
Subjects
Details
- ISSN :
- 18734235
- Volume :
- 24
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Biosensorsbioelectronics
- Accession number :
- edsair.doi.dedup.....2bfa18cd559c9eda9f4139fc0c3315b1