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Overexpression of TNKS1BP1 in lung cancers and its involvement in homologous recombination pathway of DNA double-strand breaks
- Source :
- Cancer Medicine
- Publication Year :
- 2017
- Publisher :
- Wiley, 2017.
-
Abstract
- TNKS1BP1 is a member of the poly(ADP‐ribose) polymerase (PARP) superfamily. Our previous studies have demonstrated that TNKS1BP1 plays an important role in DNA damage response. But whether and how TNKS1BP1 associates with cancer is still not clear. Here, we found that TNKS1BP1 was upregulated in human lung adenocarcinoma (LAC) tissues, and was associated with poor overall survival (OS) in LAC patients. Dysregulation of TNKS1BP1 affected the sensitivity of A549 cells to several DNA damage agents including cisplatin, bleomycin, and ionizing radiation. Mechanically, overexpression of TNKS1BP1 increased the accumulation of S phase cells, which was accompanied by a decrease in M phase cells. More importantly, we found TNKS1BP1 regulated genome stability, mainly through affecting the homologous recombination pathway of DNA double‐strand breaks by inhibiting the RAD51 foci formation. Overall, our study indicates that, in LAC, aberrant expressions of TNKS1BP1 are common events, and overexpression of TNKS1BP1 might affect outcomes of cancer patients to chemotherapy and radiotherapy.
- Subjects :
- 0301 basic medicine
Cancer Research
Lung Neoplasms
Cell Survival
DNA damage
Poly ADP ribose polymerase
RAD51
Adenocarcinoma of Lung
Adenocarcinoma
Biology
chemotherapy
Bleomycin
03 medical and health sciences
chemistry.chemical_compound
medicine
Humans
DNA Breaks, Double-Stranded
Lung adenocarcinoma (LAC)
DNA homologous recombination
Radiology, Nuclear Medicine and imaging
Telomeric Repeat Binding Protein 1
Cell Proliferation
Original Research
Cancer Biology
Cisplatin
TNKS1BP1
Recombinational DNA Repair
Cell cycle
Prognosis
Survival Analysis
Molecular biology
Up-Regulation
Homologous Recombination Pathway
Gene Expression Regulation, Neoplastic
030104 developmental biology
Oncology
chemistry
A549 Cells
cell cycle
DNA
medicine.drug
Subjects
Details
- ISSN :
- 20457634
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Cancer Medicine
- Accession number :
- edsair.doi.dedup.....2c3f219fbfb6ddb2241f1a2734811aac