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Regulation of Autoimmune Diabetes by Complete Freund’s Adjuvant Is Mediated by NK Cells
- Source :
- The Journal of Immunology. 172:937-942
- Publication Year :
- 2004
- Publisher :
- The American Association of Immunologists, 2004.
-
Abstract
- Autoimmune (type 1) diabetes results from a loss of β cells that is mediated by self-reactive T cells. Previous studies have shown that a single injection of CFA prevents diabetes in nonobese diabetic (NOD) mice, but the mechanism(s) of protection remain unknown. We show here that NOD mice immunized with CFA have a markedly reduced incidence of diabetes and that this reduced incidence is associated with a decrease in the number of β cell-specific, autoreactive CTL. In addition, the adoptive transfer of diabetes into syngeneic NOD/SCID recipients was prevented by CFA immunization, and the protective effects of CFA were lost when cells expressing the NK cell marker, asialo GM1, were removed from both donor cells and recipient mice. Returning a population of CD3−DX5+ cells to the adoptive transfer restored the protective effects of CFA. Therefore, NK cells mediate the protective effects of CFA possibly through the down-regulation of autoreactive CTL and stimulation of NK cells represents a novel approach to the prevention of autoimmune diabetes.
- Subjects :
- Adoptive cell transfer
Freund's Adjuvant
Immunology
Population
Down-Regulation
Mice, SCID
Nod
Prediabetic State
Interferon-gamma
Islets of Langerhans
Mice
Mice, Inbred NOD
Animals
Immunology and Allergy
Medicine
Interferon gamma
Lymphocyte Count
education
Cell Aggregation
NOD mice
education.field_of_study
business.industry
Adoptive Transfer
Cell aggregation
Killer Cells, Natural
CTL
Diabetes Mellitus, Type 1
Freund's adjuvant
Female
business
Spleen
T-Lymphocytes, Cytotoxic
medicine.drug
Subjects
Details
- ISSN :
- 15506606 and 00221767
- Volume :
- 172
- Database :
- OpenAIRE
- Journal :
- The Journal of Immunology
- Accession number :
- edsair.doi.dedup.....2c49f1f46c742bbb82a75ea8d56eebce