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Three-dimensional reconstruction of tissues
- Source :
- Current Protocols in Molecular Biology
- Publication Year :
- 2008
-
Abstract
- Cellular morphology is inherently three-dimensional. However, most histological techniques for tissue analysis focus on extracting information from two-dimensional slices of fixed samples or dissociated cells. These techniques result in a significant loss of the three-dimensional information of the tissue, including true cell volume, orientation, and whole cell shape. This unit discusses various options for three-dimensional imaging, provides a protocol for performing post-processing reconstruction based on serial slicing, and discusses the current advantages and limitations of the three-dimensional approach to quantitative tissue analysis. The focus of this protocol is on cardiac tissue, but the techniques can be applied to any solid tissue.
Details
- ISSN :
- 19343647
- Database :
- OpenAIRE
- Journal :
- Current protocols in molecular biology
- Accession number :
- edsair.doi.dedup.....2c72c53c34955de89456f1b467ee5318