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Haloferax volcanii Proteome Response to Deletion of a Rhomboid Protease Gene
- Source :
- Journal of proteome research. 17(3)
- Publication Year :
- 2018
-
Abstract
- Rhomboids are conserved intramembrane serine proteases involved in cell signaling processes. Their role in prokaryotes is scarcely known and remains to be investigated in Archaea. We previously constructed a rhomboid homologue deletion mutant (ΔrhoII) in Haloferax volcanii, which showed reduced motility, increased novobiocin sensitivity, and an N- glycosylation defect. To address the impact of rhoII deletion on H. volcanii physiology, the proteomes of mutant and parental strains were compared by shotgun proteomics. A total of 1847 proteins were identified (45.8% of H. volcanii predicted proteome), from which 103 differed in amount. Additionally, the mutant strain evidenced 99 proteins with altered electrophoretic migration, which suggested differential post-translational processing/modification. Integral membrane proteins that evidenced variations in concentration, electrophoretic migration, or semitryptic cleavage in the mutant were considered as potential RhoII targets. These included a PrsW protease homologue (which was less stable in the mutant strain), a predicted halocyanin, and six integral membrane proteins potentially related to the mutant glycosylation (S-layer glycoprotein, Agl15) and cell adhesion/motility (flagellin1, HVO-1153, PilA1, and PibD) defects. This study investigated for the first time the impact of a rhomboid protease on the whole proteome of an organism. Fil: Costa, Mariana Inés. Universidad Nacional de Mar del Plata; Argentina Fil: Cerletti, Micaela. Universidad Nacional de Mar del Plata; Argentina Fil: Paggi, Roberto Alejandro. Universidad Nacional de Mar del Plata; Argentina Fil: Trötschel, Christian. Ruhr Universität Bochum; Alemania Fil: de Castro, Rosana Esther. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina Fil: Poetsch, Ansgar. Ruhr Universität Bochum; Alemania Fil: Gimenez, Maria Ines. Universidad Nacional de Mar del Plata; Argentina
- Subjects :
- 0301 basic medicine
Proteases
Spectrometry, Mass, Electrospray Ionization
Glycosylation
Protease substrate
Proteome
medicine.medical_treatment
Archaeal Proteins
Mutant
Biochemistry
Substrate Specificity
Ciencias Biológicas
03 medical and health sciences
chemistry.chemical_compound
Biología Celular, Microbiología
Endopeptidases
Metalloproteins
medicine
Shotgun proteomics
Cell Adhesion
Integral membrane protein
Rhomboid protease
Haloferax volcanii
Protease
030102 biochemistry & molecular biology
biology
Chemistry
Halorerax volcanii
Membrane Proteins
Molecular Sequence Annotation
General Chemistry
biology.organism_classification
Archea
Cell biology
DNA-Binding Proteins
030104 developmental biology
Gene Ontology
Gene Expression Regulation, Archaeal
Protein Processing, Post-Translational
CIENCIAS NATURALES Y EXACTAS
Gene Deletion
Bacterial Outer Membrane Proteins
Subjects
Details
- ISSN :
- 15353907
- Volume :
- 17
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Journal of proteome research
- Accession number :
- edsair.doi.dedup.....2c8e239d893e754c49efd3842a6897e1