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IP3 mediated global Ca2+ signals arise through two temporally and spatially distinct modes of Ca2+ release
- Source :
- eLife, Vol 9 (2020)
- Publication Year :
- 2020
- Publisher :
- eLife Sciences Publications Ltd, 2020.
-
Abstract
- The ‘building-block’ model of inositol trisphosphate (IP3)-mediated Ca2+ liberation posits that cell-wide cytosolic Ca2+ signals arise through coordinated activation of localized Ca2+ puffs generated by stationary clusters of IP3 receptors (IP3Rs). Here, we revise this hypothesis, applying fluctuation analysis to resolve Ca2+ signals otherwise obscured during large Ca2+ elevations. We find the rising phase of global Ca2+ signals is punctuated by a flurry of puffs, which terminate before the peak by a mechanism involving partial ER Ca2+ depletion. The continuing rise in Ca2+, and persistence of global signals even when puffs are absent, reveal a second mode of spatiotemporally diffuse Ca2+ signaling. Puffs make only small, transient contributions to global Ca2+ signals, which are sustained by diffuse release of Ca2+ through a functionally distinct process. These two modes of IP3-mediated Ca2+ liberation have important implications for downstream signaling, imparting spatial and kinetic specificity to Ca2+-dependent effector functions and Ca2+ transport.
- Subjects :
- Physics
General Immunology and Microbiology
QH301-705.5
General Neuroscience
ip3 receptor
Science
Inositol trisphosphate
General Medicine
Inositol trisphosphate receptor
calcium signaling
General Biochemistry, Genetics and Molecular Biology
chemistry.chemical_compound
calcium imaging
Calcium imaging
Structural biology
chemistry
Biophysics
Medicine
Ca2 release
Effector functions
Biology (General)
Calcium signaling
Subjects
Details
- Language :
- English
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- eLife
- Accession number :
- edsair.doi.dedup.....2ca46e5d0c61800f2e3fc578234f2d46