Additional file 1 of Lung-gut axis of microbiome alterations following co-exposure to ultrafine carbon black and ozone

Additional file 1. Sequence data are available in NCBI database BioProject PRJNA891236. The online version contains additional file available at journal website. It includes. Figure S1: Changes in weight after single and multiple CB, O3 and CB + O3 inhalation exposures. Figure S2: Gating strategy for identifying inflammatory cells in lungs. A Flow gating strategy to detect cell types in lungs after air, CB, O3 or CB + O3 exposure either for 3 h for one day or four days. B Inflammatory cells identification markers. C Inflammatory cell markers, conjugated fluorochrome, clone and vendors. Figure S3: Exposure induce changes in bacterial community in the lungs. A Alterations in the Lactobacillus in response to inhalation exposure in the lungs. B, C Principle Co-ordinate analysis of lung microbial community, Bray–Curtis, and Unweighted Unifrac, respectively. Figure S4: Exposure induce changes in beta diversity indices in colon contents microbiome. A–C Principle Co-ordinate analysis of colon content microbial community, Bray–Curtis, Jaccard distance and Unweighted Unifrac, respectively. D Alterations in the Firmicutes to Bacteroidetes ratio in the colon content. Figure S5: Correlation analysis of absolute bacterial load in the colon contents to oxidative stress.