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Root‐type<scp>ferredoxin‐NADP</scp>+oxidoreductase isoforms in<scp>Arabidopsis thaliana</scp>: Expression patterns, location and stress responses
- Source :
- Grabsztunowicz, M, Rantala, M, Ivanauskaite, A, Blomster, T, Koskela, M M, Vuorinen, K, Tyystjarvi, E, Burow, M, Overmyer, K, Mahonen, A P & Mulo, P 2021, ' Root-type ferredoxin-NADP + oxidoreductase isoforms in Arabidopsis thaliana : Expression patterns, location and stress responses ', Plant, Cell and Environment, vol. 44, no. 2, pp. 548-558 . https://doi.org/10.1111/pce.13932
- Publication Year :
- 2020
- Publisher :
- Wiley, 2020.
-
Abstract
- In Arabidopsis, two leaf-type ferredoxin-NADP(+) oxidoreductase (LFNR) isoforms function in photosynthetic electron flow in reduction of NADP(+), while two root-type FNR (RFNR) isoforms catalyse reduction of ferredoxin in non-photosynthetic plastids. As the key to understanding, the function of RFNRs might lie in their spatial and temporal distribution in different plant tissues and cell types, we examined expression of RFNR1 and RFNR2 genes using beta-glucuronidase (GUS) reporter lines and investigated accumulation of distinct RFNR isoforms using a GFP approach and Western blotting upon various stresses. We show that while RFNR1 promoter is active in leaf veins, root tips and in the stele of roots, RFNR2 promoter activity is present in leaf tips and root stele, epidermis and cortex. RFNR1 protein accumulates as a soluble protein within the plastids of root stele cells, while RFNR2 is mainly present in the outer root layers. Ozone treatment of plants enhanced accumulation of RFNR1, whereas low temperature treatment specifically affected RFNR2 accumulation in roots. We further discuss the physiological roles of RFNR1 and RFNR2 based on characterization of rfnr1 and rfnr2 knock-out plants and show that although the function of these proteins is partly redundant, the RFNR proteins are essential for plant development and survival.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Gene isoform
Physiology
FNR
NADP(+) oxidoreductase
Arabidopsis
Plant Science
low temperature
01 natural sciences
03 medical and health sciences
Oxidoreductase
ferredoxin‐
THYLAKOID MEMBRANES
NITRITE REDUCTION
Arabidopsis thaliana
ELECTRON-TRANSFER
PLANTS
Plastid
plastid
Ferredoxin
chemistry.chemical_classification
PURIFICATION
biology
Epidermis (botany)
Chemistry
fungi
food and beverages
stress response
11831 Plant biology
root
biology.organism_classification
Cell biology
ozone
030104 developmental biology
GLUTAMATE SYNTHASE
Stele
gene expression
NADP+-OXIDOREDUCTASE
DIFFERENT ORGANS
COMPLEXES
010606 plant biology & botany
Subjects
Details
- ISSN :
- 13653040 and 01407791
- Volume :
- 44
- Database :
- OpenAIRE
- Journal :
- Plant, Cell & Environment
- Accession number :
- edsair.doi.dedup.....2cdde3bc6c6523de6dbcaeeabfaedee4