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Additional file 7 of SMYD3 associates with the NuRD (MTA1/2) complex to regulate transcription and promote proliferation and invasiveness in hepatocellular carcinoma cells

Authors :
Yang, Yang
Qiu, Rongfang
Zhao, Siyu
Shen, Lin
Tang, Bufu
Weng, Qiaoyou
Xu, Ziwei
Zheng, Liyun
Chen, Weiqian
Shu, Gaofeng
Wang, Yajie
Zhao, Zhongwei
Chen, Minjiang
Ji, Jiansong
Publication Year :
2023
Publisher :
figshare, 2023.

Abstract

Additional file 7: Fig. S3. (a) Rescue experiment of EdU incorporation assays. LM3 cells were transfected with the indicated siRNA. EdU incorporation assays were performed using a fluorescence method. Representative images and statistical analyses are shown. (b) Rescue experiment of transwell invasion assays. LM3 cells were transfected with the indicated specific siRNA. The invaded cells were stained and quantified. The images represent one field under microscopy in each group. The efficiency of protein knockdown was verified by western blotting. *P < 0.05 and **P < 0.01 (two-tailed t-test). (c) The knockdown efficiencies of SMYD3 and IGFBP4 were confirmed by western blot.

Details

Database :
OpenAIRE
Accession number :
edsair.doi.dedup.....2cef3ed5db337e1b65b107a783927d66
Full Text :
https://doi.org/10.6084/m9.figshare.22607849.v1