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Probing Bacterial Adhesion at the Single-Molecule and Single-Cell Levels by AFM-Based Force Spectroscopy
- Source :
- Methods in molecular biology, Methods in molecular biology, 1814, pp.403-414, 2018, 978-1-4939-8590-6 (print)-978-1-4939-8591-3 (online). ⟨10.1007/978-1-4939-8591-3_24⟩, Methods in Molecular Biology ISBN: 9781493985906
- Publication Year :
- 2018
-
Abstract
- Functionalization of AFM probes with biomolecules or microorganisms allows for a better understanding of the interaction mechanisms driving microbial adhesion. Here we describe the most commonly used protocols to graft molecules and bacteria to AFM cantilevers. The bioprobes obtained that way enable to measure forces down to the single-cell and single-molecule levels.
- Subjects :
- 0301 basic medicine
chemistry.chemical_classification
Materials science
Atomic force microscopy
Biomolecule
Cell
technology, industry, and agriculture
Force spectroscopy
Nanotechnology
Adhesion
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
03 medical and health sciences
030104 developmental biology
medicine.anatomical_structure
chemistry
Microbial adhesion
medicine
Molecule
Surface modification
ComputingMilieux_MISCELLANEOUS
Subjects
Details
- Language :
- English
- ISBN :
- 978-1-4939-8590-6
- ISBNs :
- 9781493985906
- Database :
- OpenAIRE
- Journal :
- Methods in molecular biology, Methods in molecular biology, 1814, pp.403-414, 2018, 978-1-4939-8590-6 (print)-978-1-4939-8591-3 (online). ⟨10.1007/978-1-4939-8591-3_24⟩, Methods in Molecular Biology ISBN: 9781493985906
- Accession number :
- edsair.doi.dedup.....2cf2f267b011bddc31b870a0b4bf5817
- Full Text :
- https://doi.org/10.1007/978-1-4939-8591-3_24⟩