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Cyanamide mediated syntheses under plausible primitive earth conditions
- Source :
- Journal of Molecular Evolution. 10:193-209
- Publication Year :
- 1977
- Publisher :
- Springer Science and Business Media LLC, 1977.
-
Abstract
- When an aqueous solution (pH 7.0) of 3H deoxythymidine 5'-triphosphate, deoxythymidine 5'-phosphate, 4-amino-5-imidazolecarboxamide, cyanamide and ammonium chloride was dried and heated at 60 degrees C for 18 h, oligomers were obtained in a yield of approximately 80%. After the chemical degradation of any pyrophosphate bonds present in these oligomers, linear polynucleotides of up to 7-8 units in length were isolated by DEAE cellulose column chromatography and identified by enzymatic digestion procedures. The di- and trinucleotide fractions were degraded 87% and 100% by snake venom phosphodiesterase and 39% and 9% by spleen phosphodiesterase. This synthesis of deoxythymidine oligonucleotides was conducted under potentially prebiotic conditions and may offer a possible method for the synthesis of deoxyoligonucleotides on the primitive Earth.
- Subjects :
- Hot Temperature
Chemical Phenomena
Oligonucleotides
Biology
Aminoimidazole Carboxamide
Pyrophosphate
Medicinal chemistry
Thin-layer chromatography
Chemistry
chemistry.chemical_compound
Paper chromatography
Column chromatography
chemistry
Polymerization
Polynucleotide
Thymidine Monophosphate
Genetics
Thymine Nucleotides
Ammonium chloride
Cyanamide
Molecular Biology
Ecology, Evolution, Behavior and Systematics
Subjects
Details
- ISSN :
- 14321432 and 00222844
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Journal of Molecular Evolution
- Accession number :
- edsair.doi.dedup.....2d27c99027e20296e4312d5a80f90ca4
- Full Text :
- https://doi.org/10.1007/bf01764595