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3D Biomimetic Scaffold for Growth Factor Controlled Delivery: An in-vitro study of Tenogenic events on Wharton’s Jelly Mesenchymal stem cells
- Source :
- Pharmaceutics, Vol 13, Iss 1448, p 1448 (2021), Pharmaceutics, Volume 13, Issue 9
- Publication Year :
- 2021
-
Abstract
- The present work described a bio-functionalized 3D fibrous construct, as an interactive teno-inductive graft model to study tenogenic potential events of human mesenchymal stem cells collected from Wharton’s Jelly (hWJ-MSCs). The 3D-biomimetic and bioresorbable scaffold was functionalized with nanocarriers for the local controlled delivery of a teno-inductive factor, i.e., the human Growth Differentiation factor 5 (hGDF-5). Significant results in terms of gene expression were obtained. Namely, the up-regulation of Scleraxis (350-fold, p ≤ 0.05), type I Collagen (8-fold), Decorin (2.5-fold), and Tenascin-C (1.3-fold) was detected at day 14<br />on the other hand, when hGDF-5 was supplemented in the external medium only (in absence of nanocarriers), a limited effect on gene expression was evident. Teno-inductive environment also induced pro-inflammatory, (IL-6 (1.6-fold), TNF (45-fold, p ≤ 0.001), and IL-12A (1.4-fold)), and anti-inflammatory (IL-10 (120-fold) and TGF-β1 (1.8-fold)) cytokine expression upregulation at day 14. The presented 3D construct opens perspectives for the study of drug controlled delivery devices to promote teno-regenerative events.
- Subjects :
- 3D fibrin scaffold
Cyclic strain bioreactor systems
HGDF-5 controlled delivery
Human Wharton’s Jelly Mesenchymal Stem Cells
PLGA nanocarriers
Tenogenic commitment
Decorin
medicine.medical_treatment
Pharmaceutical Science
Article
RS
Pharmacy and materia medica
Wharton's jelly
medicine
Chemistry
Growth factor
Mesenchymal stem cell
Scleraxis
Growth differentiation factor
Human Wharton’s Jelly Mesenchymal Stem Cell
Cell biology
RS1-441
PLGA nanocarrier
Nanocarriers
Type I collagen
Cyclic strain bioreactor system
Subjects
Details
- Language :
- English
- ISSN :
- 19994923
- Database :
- OpenAIRE
- Journal :
- Pharmaceutics, Vol 13, Iss 1448, p 1448 (2021), Pharmaceutics, Volume 13, Issue 9
- Accession number :
- edsair.doi.dedup.....2d62fb2d930594eee58c623b60cfe283