Back to Search
Start Over
Phytochemicals with NO inhibitory effects and interactions with iNOS protein from Trigonostemon howii
- Source :
- Bioorganic Chemistry. 75:71-77
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- A phytochemical investigation to obtain new NO inhibitors led to the isolation of nine compounds including one new guaiane-type sesquiterpenoid (1) and two new cleistanthane diterpenoids (2 and 3) from the stems of Trigonostemon howii. Their structures were elucidated on the basis of extensive 1D and 2D NMR spectroscopic data analysis, and the absolute configurations of new compounds 1–3 were established via comparison of experimental and calculated electronic circular dichroism (ECD) spectra. Compounds 2 and 3 possess a rare 3,4-seco-cleistanthane diterpenoid skeleton. All of the compounds showed inhibitory effects on lipopolysaccharide-induced NO production in murine microglial BV-2 cells. The further molecular docking studies indicated the strong interactions between some bioactive compounds with the iNOS protein, which revealed the possible and potential mechanism of NO inhibition of bioactive compounds.
- Subjects :
- Circular dichroism
Magnetic Resonance Spectroscopy
Stereochemistry
Phytochemicals
Molecular Conformation
Nitric Oxide Synthase Type II
Nitric Oxide
Inhibitory postsynaptic potential
01 natural sciences
Biochemistry
Sesquiterpenes, Guaiane
Catalytic Domain
Trigonostemon
Drug Discovery
Enzyme Inhibitors
No production
Inos protein
Molecular Biology
Binding Sites
Plant Stems
biology
010405 organic chemistry
Chemistry
Circular Dichroism
Organic Chemistry
Euphorbiaceae
biology.organism_classification
Terpenoid
0104 chemical sciences
Molecular Docking Simulation
010404 medicinal & biomolecular chemistry
Phytochemical
Thermodynamics
Diterpenes
Two-dimensional nuclear magnetic resonance spectroscopy
Subjects
Details
- ISSN :
- 00452068
- Volume :
- 75
- Database :
- OpenAIRE
- Journal :
- Bioorganic Chemistry
- Accession number :
- edsair.doi.dedup.....2da3aaff9f1785edf357859deedcbc30