Effects of chronic exposure of methomyl on the antioxidant system in kidney of Nile tilapia (Oreochromis niloticus) and recovery pattern

Tilapia were exposed to sublethal methomyl concentrations of 0, 0.2, 2, 20, or 200 μg/L for 30 d, and then were transferred to methomyl-free water for 18 d. Renal antioxidant parameters, including catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), glutathione peroxidase (GPx) , glutathione reductase (GR), total glutathione (GSH), and reduced glutathione (GSSG), were examined in tilapia at d 0, 6, 12, 18, 24, and 30 after starting the experiment and at 18 d after transferring to methomyl-free water. There were no significant changes in enzymatic activity and content of antioxidants in kidney of tilapia exposed to 0.2 μg/L methomyl compared to controls. The results showed significant increases in SOD, CAT, GST, GR, GPx, and level of GSSG accompanied by a decrease in GSH levels following methomyl exposure in tilapia to 2, 20, or 200 μg/L over the 30-d exposure period, suggesting the presence of oxidative stress. Thus, it would appear the 0.2 μg/L methomyl might be considered the no-observed-adverse-effect level (NOAEL). Recovery data showed that the effects produced by lower concentration of methomyl at 20 μg/L were reversible but not at the higher 200 μg/L concentration.