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Optimizing Crystal Size of Photosystem II by Macroseeding: Toward Neutron Protein Crystallography

Authors :
Holger Dobbek
Jan Kern
Athina Zouni
Vittal K. Yachandra
Junko Yano
Mohamed Ibrahim
Frank Müh
Ruchira Chatterjee
Rana Hussein
Leighton Coates
Source :
Crystal growth & design, vol 18, iss 1, Hussein, R; Ibrahim, M; Chatterjee, R; Coates, L; Müh, F; Yachandra, VK; et al.(2018). Optimizing Crystal Size of Photosystem II by Macroseeding: Toward Neutron Protein Crystallography. Crystal Growth and Design, 18(1), 85-94. doi: 10.1021/acs.cgd.7b00878. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/56m483xp
Publication Year :
2018
Publisher :
eScholarship, University of California, 2018.

Abstract

© 2017 American Chemical Society. Photosystem II (PSII) catalyzes the photo-oxidation of water to molecular oxygen and protons. The water splitting reaction occurs inside the oxygen-evolving complex (OEC) via a Mn4CaO5cluster. To elucidate the reaction mechanism, detailed structural information for each intermediate state of the OEC is required. Despite the current high-resolution crystal structure of PSII at 1.85 Å and other efforts to follow the structural changes of the Mn4CaO5cluster using X-ray free electron laser (XFEL) crystallography in addition to spectroscopic methods, many details about the reaction mechanism and conformational changes in the catalytic site during water oxidation still remain elusive. In this study, we present a rarely found successful application of the conventional macroseeding method to a large membrane protein like the dimeric PSII core complex (dPSIIcc). Combining microseeding with macroseeding crystallization techniques allowed us to reproducibly grow large dPSIIcc crystals with a size of ∼3 mm. These large crystals will help improve the data collected from spectroscopic methods like polarized extended X-ray absorption fine structure (EXAFS) and single crystal electron paramagnetic resonance (EPR) techniques and are a prerequisite for determining a three-dimensional structure using neutron diffraction.

Details

Database :
OpenAIRE
Journal :
Crystal growth & design, vol 18, iss 1, Hussein, R; Ibrahim, M; Chatterjee, R; Coates, L; Müh, F; Yachandra, VK; et al.(2018). Optimizing Crystal Size of Photosystem II by Macroseeding: Toward Neutron Protein Crystallography. Crystal Growth and Design, 18(1), 85-94. doi: 10.1021/acs.cgd.7b00878. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/56m483xp
Accession number :
edsair.doi.dedup.....2dd4bca14a109efd42c9b134b8a9fe7f
Full Text :
https://doi.org/10.1021/acs.cgd.7b00878.