Back to Search
Start Over
Optimizing Crystal Size of Photosystem II by Macroseeding: Toward Neutron Protein Crystallography
- Source :
- Crystal growth & design, vol 18, iss 1, Hussein, R; Ibrahim, M; Chatterjee, R; Coates, L; Müh, F; Yachandra, VK; et al.(2018). Optimizing Crystal Size of Photosystem II by Macroseeding: Toward Neutron Protein Crystallography. Crystal Growth and Design, 18(1), 85-94. doi: 10.1021/acs.cgd.7b00878. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/56m483xp
- Publication Year :
- 2018
- Publisher :
- eScholarship, University of California, 2018.
-
Abstract
- © 2017 American Chemical Society. Photosystem II (PSII) catalyzes the photo-oxidation of water to molecular oxygen and protons. The water splitting reaction occurs inside the oxygen-evolving complex (OEC) via a Mn4CaO5cluster. To elucidate the reaction mechanism, detailed structural information for each intermediate state of the OEC is required. Despite the current high-resolution crystal structure of PSII at 1.85 Å and other efforts to follow the structural changes of the Mn4CaO5cluster using X-ray free electron laser (XFEL) crystallography in addition to spectroscopic methods, many details about the reaction mechanism and conformational changes in the catalytic site during water oxidation still remain elusive. In this study, we present a rarely found successful application of the conventional macroseeding method to a large membrane protein like the dimeric PSII core complex (dPSIIcc). Combining microseeding with macroseeding crystallization techniques allowed us to reproducibly grow large dPSIIcc crystals with a size of ∼3 mm. These large crystals will help improve the data collected from spectroscopic methods like polarized extended X-ray absorption fine structure (EXAFS) and single crystal electron paramagnetic resonance (EPR) techniques and are a prerequisite for determining a three-dimensional structure using neutron diffraction.
- Subjects :
- 0301 basic medicine
Reaction mechanism
Photosystem II
1.1 Normal biological development and functioning
Crystal structure
Oxygen-evolving complex
010402 general chemistry
Photochemistry
01 natural sciences
Physical Chemistry
Article
law.invention
Crystal
Inorganic Chemistry
03 medical and health sciences
law
Underpinning research
General Materials Science
Crystallization
Chemistry
General Chemistry
Materials Engineering
Condensed Matter Physics
0104 chemical sciences
Crystallography
030104 developmental biology
Generic Health Relevance
X-ray crystallography
Water splitting
Inorganic & Nuclear Chemistry
Physical Chemistry (incl. Structural)
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Crystal growth & design, vol 18, iss 1, Hussein, R; Ibrahim, M; Chatterjee, R; Coates, L; Müh, F; Yachandra, VK; et al.(2018). Optimizing Crystal Size of Photosystem II by Macroseeding: Toward Neutron Protein Crystallography. Crystal Growth and Design, 18(1), 85-94. doi: 10.1021/acs.cgd.7b00878. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/56m483xp
- Accession number :
- edsair.doi.dedup.....2dd4bca14a109efd42c9b134b8a9fe7f
- Full Text :
- https://doi.org/10.1021/acs.cgd.7b00878.