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Implementation and application of a multiplex assay to detect malaria-specific antibodies : a promising tool for assessing malaria transmission in Southeast Asian pre-elimination areas
- Source :
- Malaria journal, Malaria Journal, Malaria Journal, BioMed Central, 2015, 14 (338), pp.1-14. ⟨10.1186/s12936-015-0868-z⟩, Malaria Journal, BioMed Central, 2015, 14 (1), pp.338. ⟨10.1186/s12936-015-0868-z⟩
- Publication Year :
- 2015
-
Abstract
- Background Epidemiological surveillance is a key activity in malaria control and elimination in low-transmission and pre-elimination settings. Hence, sensitive tools for estimating malaria force of infection are crucial. Serological markers might provide additional information in estimating force of infection in low-endemic areas along with classical parasite detection methods. Serological markers can be used to estimate recent, past or present malaria exposure, depending on the used markers and their half-life. Methods An assay based on 14 Plasmodium-specific peptides, one peptide specific for Anopheles gambiae saliva protein and five Plasmodium-specific recombinant proteins was developed for the MAGPIX system, assessed for its performance, and applied on blood spots from 2000 individuals collected in the Ratanakiri Province, Cambodia. Results A significant correlation for the use of 1000 and 2000 beads/antigen/well as well as for the monoplex versus multiplex assay was observed for all antigens (p
- Subjects :
- Serological markers
Anopheles gambiae
Plasmodium vivax
Antibodies, Protozoan
Serology
MESH: Aged, 80 and over
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
MESH: Child
Multiplex
Malaria, Falciparum
Child
MESH: Plasmodium falciparum
Aged, 80 and over
Immunoassay
MESH: Aged
MESH: Middle Aged
biology
MESH: Malaria, Falciparum
Middle Aged
MESH: Infant
MESH: Plasmodium vivax
3. Good health
MESH: Reproducibility of Results
Infectious Diseases
MESH: Young Adult
Child, Preschool
[SDV.IMM]Life Sciences [q-bio]/Immunology
Cambodia
MESH: Immunoassay
Adult
Adolescent
Plasmodium falciparum
Multiplex immunoassay
MESH: Parasitology
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Southeast asian
Young Adult
parasitic diseases
Malaria, Vivax
medicine
Humans
MESH: Antibodies, Protozoan
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
Seroconversion
Biology
Aged
MESH: Adolescent
MESH: Humans
Research
MESH: Cambodia
MESH: Child, Preschool
Infant
Reproducibility of Results
MESH: Malaria, Vivax
MESH: Adult
medicine.disease
biology.organism_classification
Virology
Malaria
Immunology
Parasitology
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
Human medicine
Subjects
Details
- Language :
- English
- ISSN :
- 14752875
- Database :
- OpenAIRE
- Journal :
- Malaria journal
- Accession number :
- edsair.doi.dedup.....2dd9f67b587ecf7715f273456ec28c41