Back to Search
Start Over
Analysis of Serum Biochemical Indexes, Egg Quality, and Liver Transcriptome in Laying Hens Fed Diets Supplemented with Gynostemma pentaphyllum Powder
- Source :
- Genes; Volume 12; Issue 12; Pages: 1942, Genes, Genes, Vol 12, Iss 1942, p 1942 (2021)
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Gynostemma pentaphyllum (GP), known as “southern ginseng”, can reduce the blood pressure and blood lipid levels. In this study, 300 layer chicks of one day old were divided randomly into three groups (control group (base diet), high addition group (base diet with 1% GP), and low addition group (base diet with 0.5% GP)). After 29 weeks, the growth performance, egg quality, and serum index were determined. Additionally, liver mRNA was identified using RNA-seq to investigate the molecular mechanisms. The results indicated that the serum total cholesterol and triglycerides decreased significantly in the GP addition group. The addition of GP increased the egg weight, Haugh unit and redness (a*) of the egg yolk color, and reduced the yolk cholesterol concentration. Moreover, 95 differentially expressed genes (DEGs) were screened between the control and GP addition group. GO and the KEGG analysis showed that the PPAR pathway was significantly enriched. Five fatty acid metabolism-related genes (FABP3, CYP7A1, ANKRD22, SCD1, and PCK1) were validated by qRT-PCR analysis, which confirmed the tendency of the expression. These DEGs in the PPAR pathway may be the key factors of GP affecting fatty acid metabolism. These results may provide a theoretical basis for further research and new insights into GP as a feed additive.
- Subjects :
- food.ingredient
Feed additive
Blood lipids
QH426-470
Biology
Real-Time Polymerase Chain Reaction
Article
chemistry.chemical_compound
food
Yolk
Databases, Genetic
Food Quality
Genetics
Animals
Food science
Gynostemma pentaphyllum
Genetics (clinical)
Haugh unit
chemistry.chemical_classification
Plants, Medicinal
Fatty acid metabolism
Sequence Analysis, RNA
Cholesterol
laying hens
total cholesterol
Fatty Acids
Fatty acid
biology.organism_classification
Animal Feed
Egg Yolk
Gynostemma
Liver
chemistry
Dietary Supplements
Female
Powders
serum
transcriptome
Transcriptome
Chickens
Drugs, Chinese Herbal
Subjects
Details
- ISSN :
- 20734425
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Genes
- Accession number :
- edsair.doi.dedup.....2df85b4ee4d7047f089301e890eeca5e