Back to Search
Start Over
Oxidative Modification of miR-184 Enables It to Target Bcl-xL and Bcl-w
- Source :
- Molecular Cell. 59(1):50-61
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- Summary MicroRNAs (miRNAs) are small non-coding RNAs, and they bind to complementary sequences in the three prime untranslated regions (3′ UTRs) of target mRNA transcripts, thereby inhibiting mRNA translation or promoting mRNA degradation. Excessive reactive oxygen species (ROS) can cause cell-damaging effects through oxidative modification of macromolecules leading to their inappropriate functions. Such oxidative modification is related to cancers, aging, and neurodegenerative and cardiovascular diseases. Here we report that miRNAs can be oxidatively modified by ROS. We identified that miR-184 upon oxidative modification associates with the 3′ UTRs of Bcl-xL and Bcl-w that are not its native targets. The mismatch of oxidized miR-184 with Bcl-xL and Bcl-w is involved in the initiation of apoptosis in the study with rat heart cell line H9c2 and mouse models. Our results reveal a model of ROS in regulating cellular events by oxidatively modifying miRNA.
- Subjects :
- Male
Untranslated region
bcl-X Protein
Apoptosis
Bcl-xL
Oxidative phosphorylation
Biology
Cell Line
Mice
RNA interference
microRNA
Animals
RNA, Small Interfering
3' Untranslated Regions
Molecular Biology
chemistry.chemical_classification
Reactive oxygen species
Three prime untranslated region
Myocardium
Proteins
Cell Biology
Molecular biology
Rats
Cell biology
Mice, Inbred C57BL
MicroRNAs
chemistry
biology.protein
RNA Interference
Apoptosis Regulatory Proteins
Reactive Oxygen Species
Oxidation-Reduction
Subjects
Details
- ISSN :
- 10972765
- Volume :
- 59
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Molecular Cell
- Accession number :
- edsair.doi.dedup.....2e016a0eadf2a18567998eb0e79f3e2b
- Full Text :
- https://doi.org/10.1016/j.molcel.2015.05.003