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Transcription of adenovirus and HeLa cell genes in the presence of drugs that inhibit topoisomerase I and II function
- Source :
- Nucleic Acids Research. 18:1499-1508
- Publication Year :
- 1990
- Publisher :
- Oxford University Press (OUP), 1990.
-
Abstract
- The requirements for topoisomerases in transcription of adenovirus and HeLa cell genes were analyzed using drugs that specifically inhibit either topoisomerases I or II. Cleavage of viral DNA by topoisomerases in the presence of either camptothecin or VM26 was used to determine drug concentrations that led to maximal inhibition of ligation in the cleavage and ligation step of topoisomerase I or II respectively. Inhibition of topoisomerase II with VM26 did not cause a direct reduction in transcription of adenoviral genes or HeLa cell heat shock genes. VM26 did, however, interfere with other cellular processes. It reduced nucleoside uptake into HeLa cells from the medium, and it altered the normal nuclear to cytoplasmic ratio of specific RNAs. Treatment of cells with camptothecin to inhibit topoisomerase I reduced but did not abolish transcription of viral and HeLa cell genes. Transcription mediated by both RNA polymerases I and II was reduced. Topoisomerase II did not appear to substitute for topoisomerase I in transcription since treatment of cells with VM26 and camptothecin did not reduce transcript accumulation relative to cells treated with camptothecin alone.
- Subjects :
- Genes, Viral
Transcription, Genetic
Cell
Topoisomerase-I Inhibitor
HeLa
chemistry.chemical_compound
Transcription (biology)
RNA polymerase
Genetics
medicine
Humans
Topoisomerase II Inhibitors
RNA, Messenger
Antigens, Viral, Tumor
Heat-Shock Proteins
Podophyllotoxin
Teniposide
biology
Adenoviruses, Human
Topoisomerase
Adenovirus Early Proteins
Exons
Oncogene Proteins, Viral
biology.organism_classification
Molecular biology
Kinetics
medicine.anatomical_structure
Genes
chemistry
biology.protein
Camptothecin
Topoisomerase I Inhibitors
Topoisomerase-II Inhibitor
HeLa Cells
medicine.drug
Subjects
Details
- ISSN :
- 13624962 and 03051048
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Nucleic Acids Research
- Accession number :
- edsair.doi.dedup.....2e10b0d2026bcb9606c4a7e44a2fd3b7