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Higher expression of BDNF receptor gp145trkB is associated with lower apoptosis intensity in T cell lines in multiple sclerosis
- Source :
- Journal of the Neurological Sciences. 277:65-70
- Publication Year :
- 2009
- Publisher :
- Elsevier BV, 2009.
-
Abstract
- Conflicting data exist on expression of gp145trkB, the high affinity receptor for brain-derived neurotrophic factor (BDNF), on peripheral blood immunocompetent cells in multiple sclerosis (MS). We analyzed expression of gp145trkB by western blotting and flow cytometry in myelin basic protein (MBP)- and ovalbumin (OVA)-T cell lines prepared from 12 patients with relapsing–remitting MS and 12 normal healthy subjects (NHS) and correlated it with activation-induced apoptosis. We found a higher percentage of gp145trkB-expressing MBP-T cells in MS patients than in NHS ( p = 0.011). gp145trkB was mainly expressed by CD8 + T cells to a higher extent in MS patients than in NHS ( p = 0.04). MBP-T cell lines from MS patients showed significantly lower apoptosis intensity than those from NHS ( p = 0.011). We found also a significant negative correlation between gp145trkB expression and apoptosis intensity in MS patients only ( p = 0.02). OVA-T cell lines showed a gp145trkB expression similar to that of MBP-T cell lines, with a higher expression in MS patients than NHS, and similar correlations with apoptosis intensity in MS. These findings suggest that gp145trkB is mainly expressed on T cell lines from MS patients and that the BDNF/gp145trkB axis is involved in the regulation of peripheral T cell apoptosis in MS.
- Subjects :
- Adult
Male
medicine.medical_specialty
Cell Survival
T-Lymphocytes
T cell
Blotting, Western
T cells
Apoptosis
Tropomyosin receptor kinase B
Biology
neurotrophins
Brain-derived neurotrophic factor
Cell Line
Flow cytometry
Multiple sclerosis
Apoptosis, Brain-derived neurotrophic factor, Multiple sclerosis, gp145trkB, T cells, neurotrophins
Internal medicine
medicine
Humans
Receptor, trkB
gp145trkB
medicine.diagnostic_test
Flow Cytometry
medicine.disease
Myelin basic protein
Endocrinology
medicine.anatomical_structure
Neurology
biology.protein
Female
Neurology (clinical)
CD8
Subjects
Details
- ISSN :
- 0022510X
- Volume :
- 277
- Database :
- OpenAIRE
- Journal :
- Journal of the Neurological Sciences
- Accession number :
- edsair.doi.dedup.....2e261b3fb5f187096a297dab60a43d30