Back to Search
Start Over
Development and characterization of recombinant tick-borne encephalitis virus expressing mCherry reporter protein: A new tool for high-throughput screening of antiviral compounds, and neutralizing antibody assays
- Source :
- Antiviral Research. 185:104968
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- The flavivirus, tick-borne encephalitis virus (TBEV) is transmitted by Ixodes spp. ticks and may cause severe and potentially lethal neurological tick-borne encephalitis (TBE) in humans. Studying TBEV requires the use of secondary methodologies to detect the virus in infected cells. To overcome this problem, we rationally designed and constructed a recombinant reporter TBEV that stably expressed the mCherry reporter protein. The resulting TBEV reporter virus (named mCherry-TBEV) and wild-type parental TBEV exhibited similar growth kinetics in cultured cells; however, the mCherry-TBEV virus produced smaller plaques. The magnitude of mCherry expression correlated well with progeny virus production but remained stable over
- Subjects :
- 0301 basic medicine
030106 microbiology
Antibodies, Viral
Kidney
Antiviral Agents
Virus
Cell Line
Encephalitis Viruses, Tick-Borne
law.invention
03 medical and health sciences
Neutralization Tests
law
Cricetinae
Virology
medicine
Animals
Humans
Neutralizing antibody
Pharmacology
biology
medicine.disease
biology.organism_classification
Antibodies, Neutralizing
High-Throughput Screening Assays
3. Good health
Luminescent Proteins
Flavivirus
Tick-borne encephalitis virus
030104 developmental biology
biology.protein
Recombinant DNA
Antibody
mCherry
Encephalitis, Tick-Borne
Encephalitis
Subjects
Details
- ISSN :
- 01663542
- Volume :
- 185
- Database :
- OpenAIRE
- Journal :
- Antiviral Research
- Accession number :
- edsair.doi.dedup.....2e44bcbdee123902e5aa523733911d68