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Characterization of MGMT and EGFR protein expression in glioblastoma and association with survival
- Source :
- J Neurooncol
- Publication Year :
- 2019
-
Abstract
- PURPOSE: Understanding the molecular landscape of glioblastoma (GBM) is increasingly important in the age of targeted therapy. O-6-methylguanine-DNA methyltransferase (MGMT) promoter methylation and EGFR amplification are markers that may play a role in prognostication, treatment, and/or clinical trial eligibility. Quantification of MGMT and EGFR protein expression may offer an alternative strategy towards understanding GBM. Here, we quantify baseline expression of MGMT and EGFR protein in newly diagnosed GBM samples using mass spectrometry. We correlate findings with MGMT methylation and EGFR amplification statuses and survival. METHODS: We retrospectively identified adult patients with newly diagnosed resected GBM. MGMT and EGFR protein expression were quantified using a selected reaction monitoring mass spectrometry assay. Protein levels were correlated with MGMT methylation and EGFR amplification and survival data. RESULTS: We found a statistically significant association between MGMT protein expression and promoter methylation status (p=0.02) as well as between EGFR protein expression and EGFR amplification (p
- Subjects :
- Adult
Male
Cancer Research
Methyltransferase
medicine.medical_treatment
Proteomics
Protein expression
Article
Targeted therapy
03 medical and health sciences
0302 clinical medicine
Biomarkers, Tumor
Temozolomide
Medicine
Humans
Promoter Regions, Genetic
neoplasms
Antineoplastic Agents, Alkylating
DNA Modification Methylases
Aged
Retrospective Studies
Aged, 80 and over
business.industry
Brain Neoplasms
Tumor Suppressor Proteins
Methylation
Chemoradiotherapy
DNA Methylation
Middle Aged
medicine.disease
Prognosis
Combined Modality Therapy
digestive system diseases
ErbB Receptors
Survival Rate
DNA Repair Enzymes
Neurology
Oncology
030220 oncology & carcinogenesis
Cancer research
Biomarker (medicine)
Weak association
Female
Neurology (clinical)
business
Glioblastoma
030217 neurology & neurosurgery
Follow-Up Studies
Subjects
Details
- ISSN :
- 15737373
- Volume :
- 146
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of neuro-oncology
- Accession number :
- edsair.doi.dedup.....2e4cf4877e0672fd13efcbdebb213a5c