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COPI complex isoforms are required for the early acceptance of compatible pollen grains in Arabidopsis thaliana
- Source :
- Plant Reproduction. 33:97-110
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- The Coat Protein I (COPI) complex is a seven-subunit coatomer complex consisting of the α, β, β', γ, δ, ε, and ζ proteins. In Arabidopsis thaliana, COPI is required for retrograde transport from the Golgi to the endoplasmic reticulum, Golgi maintenance, and cell plate formation. During compatible pollination, vesicle recruitment to the pollen contact point is required for pollen hydration and pollen tube penetration. Here, to identify other aspects of trafficking involved in the acceptance of compatible pollen by stigmatic papillae and to determine their roles in compatible pollination, we characterized knockout lines of several isoforms of the COPI complex, including α1-COP, γ-COP, and ε-COP. Specifically, we characterized pollen grain adherence, pollen tube penetration, and seed set in the mutants. Of the mutant lines examined, α1-cop had the most severe phenotypes, including altered compatible pollen grain adherence and tube germination and reduced seed set, whereas the other lines had milder phenotypes but visibly retarded compatible pollen acceptance. This is the first study demonstrating that COPI complex subunits are required for the acceptance of compatible pollen.
- Subjects :
- 0106 biological sciences
Arabidopsis
Pollen Tube
Plant Science
Biology
medicine.disease_cause
01 natural sciences
Coat Protein Complex I
symbols.namesake
Pollen
otorhinolaryngologic diseases
medicine
Protein Isoforms
Endoplasmic reticulum
food and beverages
Cell Biology
COPI
Cell plate
Golgi apparatus
Pollen hydration
Cell biology
Coatomer
symbols
Pollen tube
010606 plant biology & botany
Subjects
Details
- ISSN :
- 21947961 and 21947953
- Volume :
- 33
- Database :
- OpenAIRE
- Journal :
- Plant Reproduction
- Accession number :
- edsair.doi.dedup.....2e82d7c318e9bc3d5464c34d51bc13fd