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An interaction between U2AF 65 and CF I(m) links the splicing and 3' end processing machineries
- Source :
- EMBO Journal, EMBO Journal, EMBO Press, 2006, 25 (20), pp.4854-64. ⟨10.1038/sj.emboj.7601331⟩, EMBO Journal, 2006, 25 (20), pp.4854-64. ⟨10.1038/sj.emboj.7601331⟩
- Publication Year :
- 2006
- Publisher :
- HAL CCSD, 2006.
-
Abstract
- The protein factor U2 snRNP Auxiliary Factor (U2AF) 65 is an essential component required for splicing and involved in the coupling of splicing and 3' end processing of vertebrate pre-mRNAs. Here we have addressed the mechanisms by which U2AF 65 stimulates pre-mRNA 3' end processing. We identify an arginine/serine-rich region of U2AF 65 that mediates an interaction with an RS-like alternating charge domain of the 59 kDa subunit of the human cleavage factor I (CF I(m)), an essential 3' processing factor that functions at an early step in the recognition of the 3' end processing signal. Tethered functional analysis shows that the U2AF 65/CF I(m) 59 interaction stimulates in vitro 3' end cleavage and polyadenylation. These results therefore uncover a direct role of the U2AF 65/CF I(m) 59 interaction in the functional coordination of splicing and 3' end processing.
- Subjects :
- Polyadenylation
RNA Splicing
Protein subunit
Biology
Bioinformatics
Article
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
0302 clinical medicine
Protein structure
Two-Hybrid System Techniques
Splicing Factor U2AF
[SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology
Humans
snRNP
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
3' Untranslated Regions
Molecular Biology
030304 developmental biology
Ribonucleoprotein
mRNA Cleavage and Polyadenylation Factors
0303 health sciences
Cell-Free System
General Immunology and Microbiology
Three prime untranslated region
General Neuroscience
Nuclear Proteins
Protein Structure, Tertiary
Cell biology
Ribonucleoproteins
030220 oncology & carcinogenesis
RNA splicing
Protein Binding
Subjects
Details
- Language :
- English
- ISSN :
- 02614189 and 14602075
- Database :
- OpenAIRE
- Journal :
- EMBO Journal, EMBO Journal, EMBO Press, 2006, 25 (20), pp.4854-64. ⟨10.1038/sj.emboj.7601331⟩, EMBO Journal, 2006, 25 (20), pp.4854-64. ⟨10.1038/sj.emboj.7601331⟩
- Accession number :
- edsair.doi.dedup.....2e8db6ea2f7b369fa7d2bc6bc3348e68