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Isolation and Regional Localization of Cosmid Linking Clones from Human Chromosome 12
- Source :
- Genomics. 21:583-587
- Publication Year :
- 1994
- Publisher :
- Elsevier BV, 1994.
-
Abstract
- The authors have developed a new method for constructing cosmid linking libraries. The method is based on the insertion of a selection gene, {beta}-lactamase, in genomic cosmid clones containing recognition sites for rare-cutting enzymes. The selection gene is maintained as a gene cassette in a plasmid and may be excised by the enzymes NotI, SacII, SplI, MluI, BssHII, and NarI or combinations of these enzymes. Using this gene cassette and a genomic cosmid library made from a human-hamster cell line containing the human chromosome 12 as its only human component, a chromosome 12-specific NotI linking library was constructed. The NotI linking clones contained recognition sites for other rare-cutting enzymes, SacII and BssHII, at high frequency, indicating the presence of CpG islands. Thirty cosmid linking clones were regionally localized by FISH and were found to be clustered to chromosome bands 12p13, 12q13, and 12q24. 28 refs., 2 figs., 1 tab.
- Subjects :
- Genetic Linkage
Restriction Mapping
Hybrid Cells
Biology
beta-Lactamases
Cell Line
Plasmid
Gene mapping
Cricetinae
Genetics
Animals
Humans
Genomic library
Gene
In Situ Hybridization, Fluorescence
Chromosome 12
Genomic Library
Chromosomes, Human, Pair 12
Chromosome Mapping
Chromosome
DNA Restriction Enzymes
Cosmids
Molecular biology
Chromosome Banding
Gene cassette
Cosmid
Subjects
Details
- ISSN :
- 08887543
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- Genomics
- Accession number :
- edsair.doi.dedup.....2e8ef4502bc3286903fafdb498ea1428