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ART-induced biophysical and biochemical alterations of Jurkat cell membrane
- Source :
- Micron (Oxford, England : 1993). 42(1)
- Publication Year :
- 2010
-
Abstract
- Integrity of the cell membrane is a basic requirement for maintaining the biological characteristics of a cell. In this study, cell membrane as the target of drug action was investigated. CCK-8 assay suggested that Artesunate (ART) could significantly suppress the proliferation of Jurkat cells in a dose-dependent manner. Changes in the morphology and mechanics of Jurkat cells were studied by atomic force microscopy (AFM). These changes included decrease of Young's modulus (from 3.18 ± 0.54 to 1.72 ± 0.54 kPa), increase in the fluctuation of surface components, increase in shrinkage, or even the appearance of pores. The Young's modulus change was according to the F-actin protein, not the Tubulin-β or integrin β1 protein. Meanwhile, the activities of plasma membrane Ca 2+ –Mg 2+ -ATPase and Na + –K + -ATPase were also repressed following ART exposure as well as membrane potential. Western blot was used to detect Caspase 3 and Cyclin D1 protein level. The Cyclin D1 was downregulated and Caspase 3 was activated. Hence, cellular membrane represented a plausible target for ART-induced injury.
- Subjects :
- Cytoplasm
ATPase
Cell
Blotting, Western
General Physics and Astronomy
Artesunate
Down-Regulation
Caspase 3
Biology
Microscopy, Atomic Force
Jurkat cells
Cell membrane
Antimalarials
Jurkat Cells
Cyclin D1
Structural Biology
medicine
Humans
General Materials Science
Na+/K+-ATPase
Cell Size
Membrane potential
Cell Membrane
Cell Biology
Actins
Artemisinins
Elasticity
Cell biology
medicine.anatomical_structure
biology.protein
Ca(2+) Mg(2+)-ATPase
Sodium-Potassium-Exchanging ATPase
Subjects
Details
- ISSN :
- 18784291
- Volume :
- 42
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Micron (Oxford, England : 1993)
- Accession number :
- edsair.doi.dedup.....2eb14d1b18262e67bc27f180d5691668