Back to Search
Start Over
Gene Expression Profile Analysis of Human Mesenchymal Stem Cells from Herniated and Degenerated Intervertebral Discs Reveals Different Expression of Osteopontin
- Source :
- Stem Cells and Development. 24:320-328
- Publication Year :
- 2015
- Publisher :
- Mary Ann Liebert Inc, 2015.
-
Abstract
- Gene expression analysis provides an effective methodology to identify clinically relevant genes implicated in intervertebral disc (IVD) pathology. The analysis of gene profile in mesenchymal stem cells (MSCs) from human herniated IVD (H-IVD) and degenerated IVD (D-IVD) has not yet been investigated. We present in this study a characterization of MSCs isolated from clinically categorized H-IVD and D-IVD disc samples. H-IVD-MSCs and D-IVD-MSCs showed multipotent mesenchymal differentiation ability, expressing positivity for adipogenic, osteogenic, and chondrogenic markers with an immunophenotypical profile representative of MSCs. FACS analyses revealed a higher expression of CD44 in D-IVD-MSCs compared to H-IVD-MSCs. Gene expression profile revealed that most genes under investigation displayed large variations and were not significantly different in the two types of analyzed IVD-MSCs. Conversely, the gene expression of osteopontin (OPN), a protein involved in bone matrix mineralization and extracellular matrix destruction, was found markedly increased (more than 400-fold) in D-IVD-MSCs compared to H-IVD-MSCs. Moreover, the OPN protein expression was detectable only in D-IVD-MSCs, and its levels were directly related with D-IVD severity. These findings suggest that an abnormal expression of OPN in D-IVD-MSCs occurs and plays a pivotal role in the pathophysiological process of human disc degeneration. We speculate that the regulation of the OPN pathway might be a therapeutic target to counteract disc degeneration.
- Subjects :
- Adult
Male
musculoskeletal diseases
Cell Separation
Intervertebral Disc Degeneration
Gene expression
medicine
Extracellular
Humans
Osteopontin
Gene
Aged
Lumbar Vertebrae
biology
Gene Expression Profiling
CD44
Mesenchymal stem cell
Mesenchymal Stem Cells
Intervertebral disc
Cell Biology
Hematology
Anatomy
Middle Aged
musculoskeletal system
Chondrogenesis
Antigens, Differentiation
Cell biology
Hyaluronan Receptors
medicine.anatomical_structure
biology.protein
Female
Cell Division
Intervertebral Disc Displacement
Developmental Biology
Subjects
Details
- ISSN :
- 15578534 and 15473287
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- Stem Cells and Development
- Accession number :
- edsair.doi.dedup.....2f0743145b6550b655880a3fd6d2f860