Back to Search
Start Over
Cyclooxygenase-2 utilizes Jun N-terminal kinases to induce invasion, but not tamoxifen resistance, in MCF-7 breast cancer cells
- Source :
- Oncology reports. 30(3)
- Publication Year :
- 2013
-
Abstract
- Elevated cyclooxygenase-2 (COX-2) expression in breast tumors is associated with a lower survival rate in patients with estrogen receptor α (ERα)-positive tumors. We hypothesized that COX-2 reduces the survival rate of breast cancer patients with ERα-positive tumors since COX-2 increases the invasiveness of ERα-positive breast tumors and decreases tumor sensitivity to tamoxifen. Previously, we demonstrated that COX-2 stimulates the activity of protein kinase C (PKC) to increase the invasiveness of ERα-positive MCF-7 breast cancer cells and to decrease the sensitivity of MCF-7 cells to tamoxifen. High levels of COX-2 are associated with the activation of the mitogen-activated protein kinase (MAPK) family and the Akt kinase. However, it is not known whether these kinases mediate COX-2-induced invasive activity and tamoxifen resistance. In the present study, we report that COX-2 utilizes PKC to enhance the phosphorylation of Jun N-terminal kinases (JNKs), but not that of other MAPK family members or Akt. Inhibition aimed at JNKs reduced COX-2-induced invasion but not COX-2-induced tamoxifen resistance. We conclude that JNKs are essential for induced cell invasion by COX-2, but not tamoxifen resistance, in ERα-positive breast cancer cells.
- Subjects :
- Cancer Research
medicine.medical_specialty
Antineoplastic Agents, Hormonal
Blotting, Western
Estrogen receptor
Apoptosis
Breast Neoplasms
Breast cancer
Cell Movement
Internal medicine
medicine
Humans
Neoplasm Invasiveness
Phosphorylation
skin and connective tissue diseases
Protein kinase A
Protein kinase B
Protein kinase C
Protein Kinase C
Cell Proliferation
business.industry
Kinase
JNK Mitogen-Activated Protein Kinases
Cancer
Nuclear Proteins
General Medicine
medicine.disease
Gene Expression Regulation, Neoplastic
Drug Combinations
Tamoxifen
Endocrinology
Oncology
Cyclooxygenase 2
Drug Resistance, Neoplasm
Cancer research
MCF-7 Cells
Female
Proteoglycans
Collagen
Laminin
business
medicine.drug
Signal Transduction
Subjects
Details
- ISSN :
- 17912431
- Volume :
- 30
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Oncology reports
- Accession number :
- edsair.doi.dedup.....2f0ebd1f37cb7967bea643db4394b5d9