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Adjuvanted multi-epitope vaccines protect HLA-A*11:01 transgenic mice against Toxoplasma gondii
- Source :
- JCI Insight. 1
- Publication Year :
- 2016
- Publisher :
- American Society for Clinical Investigation, 2016.
-
Abstract
- We created and tested multi-epitope DNA or protein vaccines with TLR4 ligand emulsion adjuvant (gluco glucopyranosyl lipid adjuvant in a stable emulsion [GLA-SE]) for their ability to protect against Toxoplasma gondii in HLA transgenic mice. Our constructs each included 5 of our best down-selected CD8(+) T cell-eliciting epitopes, a universal CD4(+) helper T lymphocyte epitope (PADRE), and a secretory signal, all arranged for optimal MHC-I presentation. Their capacity to elicit immune and protective responses was studied using immunization of HLA-A*11:01 transgenic mice. These multi-epitope vaccines increased memory CD8(+) T cells that produced IFN-γ and protected mice against parasite burden when challenged with T. gondii. Endocytosis of emulsion-trapped protein and cross presentation of the antigens must account for the immunogenicity of our adjuvanted protein. Thus, our work creates an adjuvanted platform assembly of peptides resulting in cross presentation of CD8(+) T cell-eliciting epitopes in a vaccine that prevents toxoplasmosis.
- Subjects :
- 0301 basic medicine
biology
Helper T lymphocyte
Immunogenicity
Toxoplasma gondii
General Medicine
biology.organism_classification
Major histocompatibility complex
Virology
Epitope
3. Good health
03 medical and health sciences
030104 developmental biology
0302 clinical medicine
Immune system
Immunization
Antigen
QR180
Immunology
biology.protein
030215 immunology
Subjects
Details
- ISSN :
- 23793708
- Volume :
- 1
- Database :
- OpenAIRE
- Journal :
- JCI Insight
- Accession number :
- edsair.doi.dedup.....2f6a002c04128b778e42aa3a4ebb7501
- Full Text :
- https://doi.org/10.1172/jci.insight.85955