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An application of embryonic skin cells to repair diabetic skin wound: a wound reparation trail
- Source :
- Experimental biology and medicine (Maywood, N.J.). 239(12)
- Publication Year :
- 2014
-
Abstract
- Cell therapy has shown its power to promote diabetic chronic wound healing. However, problems of scar formation and loss of appendages have not yet been solved. Our study aims to explore the potential of using embryonic skin cells (ESkCs) to repair diabetic wounds. Circular wound was created on the back of the diabetic mice, and ESkCs stained with CM-DIL were transplanted into the wound. Wound area was recorded at the day 4, 7, 11, and 14 after transplantation. The tissue samples were obtained at week 1, 2, and 3, and the tissue sections were stained by transforming growth factor β1 (TGF-β1), TGF-β3, vascular endothelial growth factor (VEGF), and CD31. The new skin formed on the wound of the diabetic mice with ESkC treatment at week 1 but not on the wounds of the non-treatment group. The histological scores of diabetic group with ESkC treatment were significantly better than the non-treatment group ( P
- Subjects :
- Chronic wound
Male
medicine.medical_specialty
Skin wound
Cell Transplantation
General Biochemistry, Genetics and Molecular Biology
Diabetic wound
Cell therapy
Diabetes Complications
Skin Physiological Phenomena
Medicine
Animals
Skin
Microscopy
Wound Healing
integumentary system
business.industry
Histocytochemistry
Embryonic stem cell
Immunohistochemistry
Surgery
Mice, Inbred C57BL
Disease Models, Animal
Wounds and Injuries
medicine.symptom
business
Subjects
Details
- ISSN :
- 15353699
- Volume :
- 239
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Experimental biology and medicine (Maywood, N.J.)
- Accession number :
- edsair.doi.dedup.....2fb51a4971d9953f77af39b00fa9a7f0