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In Vitro and In Vivo Metabolomic Profiling after Infection with Virulent Newcastle Disease Virus
- Source :
- Viruses, Volume 11, Issue 10, Viruses, Vol 11, Iss 10, p 962 (2019)
- Publication Year :
- 2019
- Publisher :
- MDPI AG, 2019.
-
Abstract
- Newcastle disease (ND) is an acute, febrile, highly contagious disease caused by the virulent Newcastle disease virus (vNDV). The disease causes serious economic losses to the poultry industry. However, the metabolic changes caused by vNDV infection remain unclear. The objective of this study was to determine the metabolomic profiling after infection with vNDV. DF-1 cells infected with the vNDV strain Herts/33 and the lungs from Herts/33-infected specific pathogen-free (SPF) chickens were analyzed via ultra-high-performance liquid chromatography/quadrupole time-of-flight tandem mass spectrometry (UHPLC-QTOF-MS) in combination with multivariate statistical analysis. A total of 305 metabolites were found to have changed significantly after Herts/33 infection, and most of them belong to the amino acid and nucleotide metabolic pathway. It is suggested that the increased pools of amino acids and nucleotides may benefit viral protein synthesis and genome amplification to promote NDV infection. Similar results were also confirmed in vivo. Identification of these metabolites will provide information to further understand the mechanism of vNDV replication and pathogenesis.
- Subjects :
- 0301 basic medicine
Genotype
Newcastle Disease
030106 microbiology
lcsh:QR1-502
Newcastle disease virus
Virulence
Disease
Biology
Newcastle disease
lcsh:Microbiology
Article
Virus
Cell Line
Pathogenesis
03 medical and health sciences
Tandem Mass Spectrometry
UHPLC-QTOF-MS
In vivo
Virology
metabolomic analysis
Animals
Metabolomics
Lung
Poultry Diseases
Heart
virulent Newcastle disease virus
biology.organism_classification
In vitro
Specific Pathogen-Free Organisms
Metabolic pathway
in vitro and in vivo
030104 developmental biology
Infectious Diseases
Chickens
Subjects
Details
- ISSN :
- 19994915
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Viruses
- Accession number :
- edsair.doi.dedup.....3007a8da5c97a1f25b95da0e0cfd15eb
- Full Text :
- https://doi.org/10.3390/v11100962