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In vitro anti-obesity effects of sesamol mediated by adenosine monophosphate-activated protein kinase and mitogen-activated protein kinase signaling in 3T3-L1 cells
- Source :
- Food Science and Biotechnology. 26:195-200
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- Sesamol is a phenol derivative of sesame oil and a potent anti-oxidant, anti-inflammatory, anti-hepatotoxic, and anti-aging compound. We investigated the effects of sesamol on the molecular mechanisms of adipogenesis in 3T3-L1 preadipocytes. The intracellular lipid accumulation accompanied by increased extracellular release of free glycerol was decreased during differentiation on treating 3T3-L1 with sesamol. Sesamol treatment on 3T3-L1 inhibited adipogenic differentiation by down-regulating adipogenesis-related factors (C/EBPα, PPARγ, and SREBP-1). Lipid accumulation was repressed by decreasing fatty acid synthase and by up-regulating lipolysis-response genes (HSL and LPL). The molecular mechanisms of sesamol-induced inhibition in adipogenesis were mediated by increased levels of phosphorylated adenosine monophosphate-activated protein kinase and its substrate acetyl-CoA carboxylase. Sesamol treatment, in turn, modulated the different members of the mitogenactivated protein kinase family by suppressing phosphorylation of ERK 1/2 and JNK and by increasing the phosphorylation of p38. In summary, sesamol inhibits adipogenic differentiation by reducing phosphorylation levels of ERK 1/2 and JNK while inducing lipolysis by activating p38 and AMPK. Our results demonstrate that the molecular mechanisms of in vitro anti-obesity effects of sesamol are due to the combined effects of preventing both lipid accumulation and adipogenesis.
- Subjects :
- 0301 basic medicine
MAPK/ERK pathway
Adenosine monophosphate
biology
Chemistry
p38 mitogen-activated protein kinases
030209 endocrinology & metabolism
Applied Microbiology and Biotechnology
Article
Cell biology
03 medical and health sciences
chemistry.chemical_compound
030104 developmental biology
0302 clinical medicine
Biochemistry
Adipogenesis
Mitogen-activated protein kinase
biology.protein
Phosphorylation
Protein kinase A
Sesamol
Food Science
Biotechnology
Subjects
Details
- ISSN :
- 20926456 and 12267708
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- Food Science and Biotechnology
- Accession number :
- edsair.doi.dedup.....3011cdcae8cb4fb8721bca59bea136d3