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Liver and serum expression of matrix metalloproteinases in asymptomatic pediatric liver transplant recipients
- Source :
- Transplant international : official journal of the European Society for Organ Transplantation. 30(2)
- Publication Year :
- 2016
-
Abstract
- We related hepatic gene and serum expression of matrix metalloproteinases (MMP) and their tissue inhibitors (TIMP) to liver histology in pediatric LT recipients. Liver biopsies and serum samples were obtained from 52 patients 10.6 years post-LT and age-matched controls for analyses of MMPs and TIMPs. Patients with fibrosis had significantly higher hepatic gene expression of MMP-2, MMP-9, MMP-14, TIMP-1, and TIMP-2 than patients without. Expression of these genes correlated with graft Metavir fibrosis stage (r = 0.494-0.684, P ≤ 0.006 for all). Gene expression of MMP-1, MMP-3, MMP-8, TIMP-3, and TIMP-4 was undetectable in both patients and controls. Portal inflammation and cytokeratin 7 correlated positively with gene expression of TIMP-1. Gene expression of MMP-2, MMP-9, and TIMP-2 correlated negatively with the time of low-dose cortisone usage (r = -0.448 to -0.422, P < 0.05 for all). Serum concentrations of MMP-8 and TIMP-1 were significantly increased and MMP-9 decreased among patients compared with controls, but no correlations to graft histology or gene expression were observed. Hepatic gene expression of certain MMPs and TIMPs is increased in stable pediatric LT recipients displaying graft fibrosis, but this did not reflect to their serum concentrations. Increased hepatic gene expression of TIMP-1 correlated with graft fibrosis stage, inflammation, and chronic cholestasis.
- Subjects :
- 0301 basic medicine
Adult
Male
medicine.medical_specialty
Pathology
Cirrhosis
Adolescent
medicine.medical_treatment
Gene Expression
Matrix metalloproteinase
Liver transplantation
Gastroenterology
03 medical and health sciences
Cytokeratin
Young Adult
0302 clinical medicine
Fibrosis
Internal medicine
Gene expression
medicine
Humans
Child
Immunosuppression Therapy
Transplantation
business.industry
Keratin-7
Histology
Tissue Inhibitor of Metalloproteinases
medicine.disease
Matrix Metalloproteinases
Liver Transplantation
030104 developmental biology
Cross-Sectional Studies
Liver
Hepatic stellate cell
030211 gastroenterology & hepatology
Female
business
Subjects
Details
- ISSN :
- 14322277
- Volume :
- 30
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Transplant international : official journal of the European Society for Organ Transplantation
- Accession number :
- edsair.doi.dedup.....3021966ef0e30c3488d3dfef45b45b71