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Hematoma size as major modulator of the cellular immune system after experimental intracerebral hemorrhage
- Source :
- Neuroscience Letters. 490:170-174
- Publication Year :
- 2011
- Publisher :
- Elsevier BV, 2011.
-
Abstract
- Inflammatory cascades are increasingly recognized as an important pathophysiological mechanism in intracerebral hemorrhage (ICH). In contrast, the effect of ICH on the systemic immune system has barely been investigated. We examined the effects of different hematoma volumes on immune cell subpopulations in experimental murine ICH. In C57BL/6 mice, ICH was induced by striatal injection of autologous blood (10, 30 or 50 μL). Control animals received the respective sham operation. Three days after ICH induction, differential blood leukocyte counting was performed. Lymphocyte subpopulations were further characterized by flow cytometry in blood, spleen, lymph node and thymus. Infectious complications were studied using microbiological cultures of blood and lungs. Only after large ICH a marked decrease of leukocyte counts and most lymphocyte subsets was observed in all organs. Despite this general leukocytopenia, a significant, up to 10-fold increase, was detected in the monocyte population after extensive hemorrhage. After moderate ICH induction, only specific lymphocyte subpopulations were differentially affected. Mature thymic cells were unaffected while immature CD4+CD8+ cells were depleted by over 90% after large ICH. A significant proportion of mice with extensive ICH (36.4%) developed spontaneous pneumonia and/or bacteremia while none of the sham operated mice had infectious complications. The ICH size determines the extent of systemic immunomodulation. Large ICH predisposes animals to infections.
- Subjects :
- CD4-Positive T-Lymphocytes
Male
Pathology
medicine.medical_specialty
Population
Spleen
Thymus Gland
Mice
Immune system
Leukocytopenia
Antigens, CD
medicine
Animals
Lymphocytes
cardiovascular diseases
education
Lymph node
Cerebral Hemorrhage
Intracerebral hemorrhage
Hematoma
Immunity, Cellular
education.field_of_study
Blood Cells
business.industry
General Neuroscience
Monocyte
Flow Cytometry
medicine.disease
Corpus Striatum
Pathophysiology
nervous system diseases
Mice, Inbred C57BL
Disease Models, Animal
medicine.anatomical_structure
Immunology
business
Subjects
Details
- ISSN :
- 03043940
- Volume :
- 490
- Database :
- OpenAIRE
- Journal :
- Neuroscience Letters
- Accession number :
- edsair.doi.dedup.....302c104d7ba5eef1a499eda3fd10ea66
- Full Text :
- https://doi.org/10.1016/j.neulet.2010.11.065