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Oxyberberine Prevented Lipopolysaccharide-Induced Acute Lung Injury through Inhibition of Mitophagy
- Source :
- Oxidative Medicine and Cellular Longevity, Oxidative Medicine and Cellular Longevity, Vol 2021 (2021)
- Publication Year :
- 2021
- Publisher :
- Hindawi, 2021.
-
Abstract
- Acute lung injury (ALI) is a serious respiratory syndrome characterized with uncontrolled inflammatory response. Oxyberberine has strong potential for clinical usage since it showed strong anti-inflammatory, antifungal, and antiarrhythmic effects in various diseases. In the present study, we evaluated whether oxyberberine can inhibit lipopolysaccharide- (LPS-) induced ALI in vivo and further evaluated the possible involvement of mitophagy in vitro by using A549 cells, a human lung epithelial cell line. Our in vivo study shows that oxyberberine significantly inhibited LPS-induced lung pathological injury and lung edema, as indicated by the changes in lung wet/dry ratio and total protein levels in the BALF in mice. Moreover, oxyberberine inhibited inflammation, as indicated by the changes of neutrophil accumulation and production of proinflammatory cytokines including tumor necrosis factor α (TNF-α), interleukin 1β (IL-1β), and IL-6 in both the lung and bronchoalveolar lavage fluid (BALF) in ALI mice. Our in vitro study shows that LPS significantly decreased the protein level of mitochondrial proteins, including cytochrome c oxidase subunit IV (COX IV), p62, and mitofusin-2 (Mfn2) in A549 cells. In addition, LPS induced significant Parkin1 translocation from cytoplasm to mitochondria. These changes were significantly inhibited by oxyberberine. Notably, the inhibitory effect of oxyberberine was almost totally lost in the presence of lysosome fusion inhibitor bafilomycin A1 (Baf), a mitophagy inhibitor. In conclusion, the present study demonstrated that oxyberberine alleviated LPS-induced inflammation in ALI via inhibition of Parkin-mediated mitophagy.
- Subjects :
- Lipopolysaccharides
Male
Aging
Lipopolysaccharide
Berberine
Article Subject
Neutrophils
Acute Lung Injury
Inflammation
Apoptosis
Pharmacology
Lung injury
Biochemistry
Proinflammatory cytokine
chemistry.chemical_compound
In vivo
Mitophagy
medicine
Animals
Edema
Humans
Lung
A549 cell
Mice, Inbred BALB C
QH573-671
medicine.diagnostic_test
Cell Biology
General Medicine
respiratory system
respiratory tract diseases
Bronchoalveolar lavage
chemistry
A549 Cells
Macrolides
medicine.symptom
Cytology
Reactive Oxygen Species
Bronchoalveolar Lavage Fluid
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19420900
- Database :
- OpenAIRE
- Journal :
- Oxidative Medicine and Cellular Longevity
- Accession number :
- edsair.doi.dedup.....3075e005b965eafb9481982b6d92768a
- Full Text :
- https://doi.org/10.1155/2021/6675264